FlyBase Allele Report: Dmel\Phb2[UAS.Tag:FLAG]

General Information

Symbol

Dmel\Phb2^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0371840

Feature type

allele

Associated gene

Dmel\Phb2

Associated Insertion(s)

Carried in Construct

P{UAS-flag.Phb2}

Key Links

Transgenic product class

cDNA

(Zhao et al., 2022)

wild_type

(Zhao et al., 2022)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zhao et al., 2022)

wild_type

(Zhao et al., 2022)

Mutagen

in vitro construct

(Zhao et al., 2022)

Progenitor genotype

Carried in construct

P{UAS-flag.Phb2}

Cytology

Description

UASt regulates expression of Phb2 cDNA (LD46344) tagged at the N-terminus with Tag:FLAG.

(Zhao et al., 2022)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Phb2

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | adult stage, with Scer\GAL4^esg.PU

(Zhao et al., 2022)

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU

(Zhao et al., 2022)

Phenotype Manifest In

adult posterior midgut epithelium, with Scer\GAL4^esg.PU

(Zhao et al., 2022)

Detailed Description

Statement

Reference

Adulthood-only expression of Phb2^UAS.Tag:FLAG under the control of Scer\GAL4^esg.PU leads to an increase in the number of intestinal progenitors (esg-positive ISCs and EBs) and an increased in the mitotic index in the adult posterior midgut.

(Zhao et al., 2022)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Phb2^UAS.Tag:FLAG/Scer\GAL4^Act5C.PI is a non-enhancer of decreased cell number | adult stage | somatic clone phenotype of Phb1²⁰

(Zhao et al., 2022)

Suppressor of

Statement

Reference

Phb2^UAS.Tag:FLAG/Scer\GAL4^Act5C.PI is a suppressor | partially of decreased cell number | somatic clone | adult stage phenotype of Raf¹¹

(Zhao et al., 2022)

Phb2^UAS.Tag:FLAG/Scer\GAL4^Act5C.PI is a suppressor | partially of decreased cell number | somatic clone | adult stage phenotype of dind^Δ66

(Zhao et al., 2022)

Phb2^UAS.Tag:FLAG/Scer\GAL4^Act5C.PI is a suppressor | partially of decreased cell number | somatic clone | adult stage phenotype of dind^Δ9

(Zhao et al., 2022)

NOT Suppressor of

Statement

Reference

Phb2^UAS.Tag:FLAG/Scer\GAL4^Act5C.PI is a non-suppressor of decreased cell number | somatic clone | adult stage phenotype of Phb1²⁰

(Zhao et al., 2022)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Phb2^UAS.Tag:FLAG/Scer\GAL4^Act5C.PI is a non-enhancer of adult posterior midgut epithelium | somatic clone phenotype of Phb1²⁰

(Zhao et al., 2022)

Suppressor of

Statement

Reference

Phb2^UAS.Tag:FLAG/Scer\GAL4^Act5C.PI is a suppressor | partially of adult posterior midgut epithelium | somatic clone phenotype of Raf¹¹

(Zhao et al., 2022)

Phb2^UAS.Tag:FLAG/Scer\GAL4^Act5C.PI is a suppressor | partially of adult posterior midgut epithelium | somatic clone phenotype of dind^Δ66

(Zhao et al., 2022)

Phb2^UAS.Tag:FLAG/Scer\GAL4^Act5C.PI is a suppressor | partially of adult posterior midgut epithelium | somatic clone phenotype of dind^Δ9

(Zhao et al., 2022)

NOT Suppressor of

Statement

Reference

Phb2^UAS.Tag:FLAG/Scer\GAL4^Act5C.PI is a non-suppressor of adult posterior midgut epithelium | somatic clone phenotype of Phb1²⁰

(Zhao et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Phb2^UAS.Tag:FLAG/Scer\GAL4^Act5C.PI rescues Phb2^Δ

(Zhao et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Phb2^UAS.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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