FlyBase Allele Report: Dmel\Pex16[CR70194-KO-kG4]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\Pex16^{CR70194-KO-kG4}

General Information

Symbol

Dmel\Pex16^{CR70194-KO-kG4}

Species

D. melanogaster

Name

FlyBase ID

FBal0375638

Feature type

allele

Associated gene

Associated Insertion(s)

TI{KozakGAL4}Pex16^{CR70194-KO-kG4}

Carried in Construct

Key Links

Genomic Maps

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

(Gene Disruption Project members, 2018-)

gene targeting by homologous recombination

(Gene Disruption Project members, 2018-)

Progenitor genotype

Associated Insertion(s)

TI{KozakGAL4}Pex16^{CR70194-KO-kG4}

Cytology

Description

A TI{KozakGAL4} DNA cassette has been inserted into Pex16, replacing the coding sequence (coordinates of deleted sequence are 3L:20777041..20778206 , release 6 genome). This results in a simultaneous knock-out of Pex16 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of Pex16 (predicted to gene trap all annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: ATAAAAATAATGAGGTGTTTCGG and TAGAGCGTTAGTATTCCCCTAGG.

(Gene Disruption Project members, 2018-)

Allele components

Component

Use(s)

Inserted element

Encoded product / tool

binary expression system - driver

Also carries

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

3L:20,777,041..20,778,206 [-]

(Gomez et al., 2025, Kanca et al., 2022, Gene Disruption Project members, 2018-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage (with Pex16¹)

(Gomez et al., 2025)

abnormal size | adult stage (with Pex16¹)

(Gomez et al., 2025)

bang sensitive (with Pex16¹)

(Gomez et al., 2025)

decreased rate of adult locomotory behavior (with Pex16¹)

(Gomez et al., 2025)

partially lethal (with Pex16¹)

(Gomez et al., 2025)

short lived (with Pex16¹)

(Gomez et al., 2025)

Phenotype Manifest In

adult motor neuron (with Pex16¹)

(Gomez et al., 2025)

axon | adult stage (with Pex16¹)

(Gomez et al., 2025)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Pex16¹/Pex16^{CR70194-KO-kG4} has short lived phenotype, non-enhanceable by Hsap\PEX16^{R176term.UAS.GFP}

(Gomez et al., 2025)

Suppressed by

Statement

Reference

Pex16¹/Pex16^{CR70194-KO-kG4} has decreased rate of adult locomotory behavior phenotype, suppressible | partially by Hsap\PEX16^UAS.GFP

(Gomez et al., 2025)

Pex16¹/Pex16^{CR70194-KO-kG4} has decreased rate of adult locomotory behavior phenotype, suppressible | partially by Hsap\PEX16^{F332del.UAS.GFP}

(Gomez et al., 2025)

Pex16¹/Pex16^{CR70194-KO-kG4} has increased mortality during development phenotype, suppressible by Hsap\PEX16^UAS.GFP

(Gomez et al., 2025)

Pex16¹/Pex16^{CR70194-KO-kG4} has increased mortality during development phenotype, suppressible by Hsap\PEX16^{F332del.UAS.GFP}

(Gomez et al., 2025)

Pex16¹/Pex16^{CR70194-KO-kG4} has short lived phenotype, suppressible | partially by Hsap\PEX16^UAS.GFP

(Gomez et al., 2025)

Pex16¹/Pex16^{CR70194-KO-kG4} has short lived phenotype, suppressible | partially by Hsap\PEX16^{F332del.UAS.GFP}

(Gomez et al., 2025)

Pex16¹/Pex16^{CR70194-KO-kG4} has bang sensitive phenotype, suppressible by Hsap\PEX16^UAS.GFP

(Gomez et al., 2025)

Pex16¹/Pex16^{CR70194-KO-kG4} has bang sensitive phenotype, suppressible by Hsap\PEX16^{F332del.UAS.GFP}

(Gomez et al., 2025)

NOT suppressed by

Statement

Reference

Pex16¹/Pex16^{CR70194-KO-kG4} has partially lethal phenotype, non-suppressible by Hsap\PEX16^{R176term.UAS.GFP}

(Gomez et al., 2025)

Pex16¹/Pex16^{CR70194-KO-kG4} has short lived phenotype, non-suppressible by Hsap\PEX16^{R176term.UAS.GFP}

(Gomez et al., 2025)

Pex16¹/Pex16^{CR70194-KO-kG4} has bang sensitive phenotype, non-suppressible by Hsap\PEX16^{R176term.UAS.GFP}

(Gomez et al., 2025)

Pex16¹/Pex16^{CR70194-KO-kG4} has decreased rate of adult locomotory behavior phenotype, non-suppressible by Hsap\PEX16^{R176term.UAS.GFP}

(Gomez et al., 2025)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

94998

y¹ w^*; TI{KozakGAL4}Pex16^{CR70194-KO-kG4}/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pex16^{CR70194-KO-kG4}

Pex16^KZ

(Gomez et al., 2025)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections