20XUAS regulatory sequences drive expression of the wild-type Gba1b coding sequence (minigene comprised of exons 1-9 including signal sequence, omitting introns and UTRs). Sequence containing a NotI site, a 6xGly linker and a Tag:V5 tag have been inserted at the C-terminal end of the open reading frame; a cloning error resulted in a frameshift after the NotI linker, rendering the Tag:V5 tag unusable.
Scer\GAL4GMR86E01/Gba1b20xUAS.cVa fails to rescue Gba1bΔ1/Df(3R)GBA1ΔTT
Scer\GAL4Pdf.PU/Gba1b20xUAS.cVa fails to rescue Gba1bΔ1/Df(3R)GBA1ΔTT