FlyBase Allele Report: Scer\GAL4[GMR56F03]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Scer\GAL4^GMR56F03

General Information

Symbol

Scer\GAL4^GMR56F03

Species

S. cerevisiae

Name

FlyBase ID

FBal0253673

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{GMR56F03-GAL4}

Also Known As

R56F03-Gal4, GMR56F03-GAL4, 56F03-GAL4, GMR56F03, Ensheathing glia-Gal4, EGN-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2011.2.9)

Progenitor genotype

Carried in construct

P{GMR56F03-GAL4}

Cytology

Description

A 2644 base pair fragment from the flanking non-coding or intronic region of CG9657 is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- larval stage

embryonic/larval ensheathing glial cell

(Kremer et al., 2017)

pupal stage -- adult stage

adult ensheathing glial cell

(Bajar et al., 2022)

pharate adult stage P15

adult ensheathing glial cell

Comment: 96 hr APF

(Wu et al., 2017)

adult ensheathing glial cell

(Hill et al., 2019)

adult synaptic neuropil ensheathing glial cell

(Kremer et al., 2017)

Additional Information

Statement

Reference

ScerGAL4^GMR56F03 drives expression in ensheathing glia at from mid-pupal through adult stages.

(Bajar et al., 2022)

ScerGAL4^GMR56F03 drives expression in neuropil ensheathing glia; expression not associated with axon tracts. ScerGAL4^GMR56F03 also drives expression in ensheathing glia in the embryonic and larval CNS.

(Kremer et al., 2017)

Drives expression in ensheathing glial cells at all stages.

(FlyBase Curators, 2017)

Marker for

ensheathing glial cell

(Bajar et al., 2022)

Reflects expression of

(Sheng et al., 2020)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number, with pros^JF02308

(Casas-Tinto et al., 2025)

abnormal circadian rhythm, with Gba1b^TKO.GS00895, Scer\GAL4^GMR85G01, Spyo\Cas9^UAS.P2

(Vaughen et al., 2022)

abnormal circadian rhythm | adult stage, with Cpes^RNAi.UAS.IR1

(Chen et al., 2022)

abnormal circadian rhythm | adult stage, with Nos^GD12013

(Kozlov et al., 2020)

abnormal heat stress response | adult stage, with sei^JF01474

(Hill et al., 2019)

abnormal neuroanatomy | adult stage, with Gba1b^TKO.GS00895, Scer\GAL4^GMR85G01, Spyo\Cas9^UAS.P2

(Vaughen et al., 2022)

abnormal neuroanatomy | adult stage | conditional, with drpr^HMS01623

(Leier et al., 2025, Nelson et al., 2024)

abnormal neuroanatomy | adult stage | conditional, with drpr^RNAi.I.UAS

(Leier et al., 2025)

abnormal neuroanatomy | adult stage | conditional, with LpR1^JF02551

(Alassaf et al., 2025)

abnormal sleep | adult stage, with Gba1b^TKO.GS00895, Scer\GAL4^GMR85G01, Spyo\Cas9^UAS.P2

(Vaughen et al., 2022)

decreased sleep | adult stage, with Gba1b^TKO.GS00895, Scer\GAL4^GMR85G01, Spyo\Cas9^UAS.P2

(Vaughen et al., 2022)

partially lethal - majority die | male, with dths^HMS03377

(Zhang et al., 2024)

viable, with Lgr4^JF03070

(Miao et al., 2024)

viable | female, with dths^HMS03377

(Zhang et al., 2024)

Phenotype Manifest In

adult brain, with Gba1b^TKO.GS00895, Scer\GAL4^GMR85G01, Spyo\Cas9^UAS.P2

(Vaughen et al., 2022)

adult ensheathing glial cell | conditional, with drpr^HMS01623

(Leier et al., 2025)

adult ensheathing glial cell | conditional, with drpr^RNAi.I.UAS

(Leier et al., 2025)

adult olfactory receptor neuron Or42a | conditional, with drpr^HMS01623

(Leier et al., 2025, Nelson et al., 2024)

adult optic lobe, with Gba1b^HMS01893, Scer\GAL4^GMR85G01

(Vaughen et al., 2022)

adult s-LNv neuron | adult stage, with Gba1b^TKO.GS00895, Scer\GAL4^GMR85G01, Spyo\Cas9^UAS.P2

(Vaughen et al., 2022)

antennal lobe glomerulus VM7 | adult stage | conditional, with drpr^HMS01623

(Leier et al., 2025)

antennal lobe glomerulus VM7 | adult stage | conditional, with drpr^RNAi.I.UAS

(Leier et al., 2025)

antennal lobe glomerulus VM7 | conditional, with drpr^HMS01623

(Nelson et al., 2024)

axon | adult stage | conditional, with drpr^HMS01623

(Leier et al., 2025)

axon | adult stage | conditional, with LpR1^JF02551

(Alassaf et al., 2025)

central nervous system, with pros^JF02308

(Casas-Tinto et al., 2025)

lysosome | adult stage, with Gba1b^HMS01893, Scer\GAL4^GMR85G01

(Vaughen et al., 2022)

lysosome | adult stage, with Gba1b^TKO.GS00895, Scer\GAL4^GMR85G01, Spyo\Cas9^UAS.P2

(Vaughen et al., 2022)

presynapse | adult stage | conditional, with drpr^HMS01623

(Leier et al., 2025)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^GMR56F03/Gba1b^{20xUAS.Tag:TM(Lamp1),Tag:V5} rescues Gba1b^Δ1/Df(3R)GBA1^ΔTT

(Vaughen et al., 2022)

Scer\GAL4^GMR56F03/Gba1b^20xUAS.cVa rescues Gba1b^Δ1/Df(3R)GBA1^ΔTT

(Vaughen et al., 2022)

Scer\GAL4^GMR56F03, Scer\GAL4^GMR85G01, Gba1b^20xUAS.cVa rescues Gba1b^Δ1/Df(3R)GBA1^ΔTT

(Vaughen et al., 2022)

Fails to rescue

Scer\GAL4^GMR56F03/Cpes^UAS.Tag:V5 fails to rescue Cpes¹

(Chen et al., 2022)

Scer\GAL4^GMR56F03/Gba1b^E404K.20xUAS fails to rescue Gba1b^Δ1/Df(3R)GBA1^ΔTT

(Vaughen et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

39157

w¹¹¹⁸; P{GMR56F03-GAL4}attP2

77469

w^*; P{GMR56F03-GAL4}attP24/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^GMR56F03

Name Synonyms

Secondary FlyBase IDs

References (54)

Report Sections