FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Gba1b
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General Information
Symbol
Dmel\Gba1b
Species
D. melanogaster
Name
Glucocerebrosidase 1b
Annotation Symbol
CG31414
Feature Type
protein_coding_gene
FlyBase ID
FBgn0051414
Gene Model Status
Current
Stock Availability
22 publicly available
Enzyme Name (EC)
glucosylceramidase (3.2.1.45)
Gene Summary
Glucocerebrosidase 1b (Gba1b) encodes a lysosomal acid beta glucocerebrosidase that catalyzes the hydrolysis of glucosylceramide to ceramide and glucose. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

dGBA1b

Key Links
Genomic Location
Cytogenetic map
95A9-95A10
Sequence location
Recombination map
3-80
RefSeq locus
NT_033777 REGION:23704804..23708512
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables glucosylceramidase activity
inferred from mutant phenotype
(Wang et al., 2022, Cabasso et al., 2019)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables glucosylceramidase activity
inferred from electronic annotation with InterPro:IPR001139
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN001667738
(GO Reference Genome Project, 2011-)
enables glucosyltransferase activity
inferred from sequence or structural similarity with UniProtKB:P04062
(Gene Ontology Curators, 2002-)
Biological Process (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in adult locomotory behavior
inferred from genetic interaction with FLYBASE:Gba1a; FB:FBgn0051148
(Davis et al., 2016)
involved_in cellular response to unfolded protein
inferred from genetic interaction with FLYBASE:Gba1a; FB:FBgn0051148
(Maor et al., 2013)
involved_in glucosylceramide catabolic process
inferred from mutant phenotype
(Wang et al., 2022)
involved_in memory
inferred from genetic interaction with FLYBASE:Gba1a; FB:FBgn0051148
(Davis et al., 2016)
involved_in neuron cellular homeostasis
inferred from genetic interaction with FLYBASE:Gba1a; FB:FBgn0051148
(Davis et al., 2016)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in glucosylceramide catabolic process
inferred from biological aspect of ancestor with PANTHER:PTN001667738
(GO Reference Genome Project, 2011-)
involved_in sphingolipid metabolic process
inferred from electronic annotation with InterPro:IPR001139
(InterPro Project Members, 2004-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in lysosome
inferred from sequence or structural similarity with UniProtKB:P04062
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
glucosylceramidase activity
a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing- 4-enine + D-glucose (3.2.1.45)
RHEA 13269:
Summaries
Gene Snapshot
Glucocerebrosidase 1b (Gba1b) encodes a lysosomal acid beta glucocerebrosidase that catalyzes the hydrolysis of glucosylceramide to ceramide and glucose. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
GLUCOSYLCERAMIDASES -
Glucosylceramidases (Glycoside hydrolase family 30) are lysosomal enzymes that catalyze the hydrolysis of glycolipid glucosylceramide (also called glucocerebroside) into simple fat ceramide and glucose. (Adapted from FBrf0230029).
UNCLASSIFIED GLUCOSYLTRANSFERASES -
This group comprises glucosyltransferases that do not classify under other groups in FlyBase.
Summary (Interactive Fly)

Gba1b mutants revealed dysregulation of proteins involved in extracellular vesicle (EV) biology, and altered protein composition of EVs was found in Gba1b mutants - Gba1b mutant protein aggregation may depend on relative depletion of specific ceramide species often enriched in EVs - human mutant GCase contributes to accumulation and aggregation of α-synuclein - In the fly, this aggregation leads to development of more severe parkinsonian signs - mutant flies showed activation of the Unfolded Protein Response (UPR) and presented inflammation and neuroinflammation that culminated in neuronopathic disease - model for Gaucher's disease

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Gba1b for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q8IMY3)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Gba1b-RB
FBtr0113405
NM_001104418
2030
566
Gba1b-RC
FBtr0334767
NM_001275951
2335
566
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Gba1b-PB
FBpp0112317
64.9
566
6.80
Q8IMY3
NP_001097888
AAN13955
Gba1b-PC
FBpp0306807
64.9
566
6.80
Q8IMY3
NP_001262880
AGB96260
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

566 aa isoforms: Gba1b-PB, Gba1b-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gba1b using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.65

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Gba1b in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 9 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 19 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Gba1b
Transgenic constructs containing regulatory region of Gba1b
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(3R)Exel6195
(Ryder, 2004.4.26)
Gene partially disrupted in
Df(3R)GBA1ΔTT
(Davis et al., 2016)
Inferred from location ( 8 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
viable (with Df(3R)GBA1ΔTT)
Other Phenotypes
Allele
abnormal circadian rhythm, with Scer\GAL4GMR56F03, Scer\GAL4GMR85G01, Spyo\Cas9UAS.P2
abnormal circadian rhythm, with Scer\GAL4repo, Spyo\Cas9UAS.P2
abnormal circadian rhythm (with Df(3R)GBA1ΔTT)
abnormal feeding behavior | adult stage
abnormal immune response | adult stage
abnormal locomotor behavior | adult stage
abnormal locomotor behavior | adult stage, with Scer\GAL4elav.PU
abnormal locomotor behavior | adult stage | progressive
abnormal neuroanatomy | adult stage, with Gba1bΔ1/Df(3R)GBA1ΔTT, Scer\GAL4Pdf.PU
abnormal neuroanatomy | adult stage, with Scer\GAL4GMR56F03, Scer\GAL4GMR85G01, Spyo\Cas9UAS.P2
abnormal neuroanatomy | adult stage (with Df(3R)GBA1ΔTT)
abnormal neuroanatomy | adult stage (with Df(3R)GBA1ΔTT), with Gba1b20xUAS.cVa, Scer\GAL4Pdf.PU
abnormal oxidative stress response | adult stage
abnormal sleep
abnormal sleep, with Scer\GAL4elav.PU
abnormal sleep | adult stage, with Scer\GAL4GMR56F03, Scer\GAL4GMR85G01, Spyo\Cas9UAS.P2
abnormal sleep | adult stage, with Scer\GAL4GMR85G01, Spyo\Cas9UAS.P2
abnormal sleep | adult stage, with Scer\GAL4repo, Spyo\Cas9UAS.P2
abnormal sleep | adult stage (with Df(3R)GBA1ΔTT)
abnormal starvation stress response | adult stage
decreased fecundity | female | progressive | recessive
decreased rate of adult locomotory behavior
decreased sleep | adult stage, with Scer\GAL4GMR56F03, Scer\GAL4GMR85G01, Spyo\Cas9UAS.P2
decreased sleep | adult stage, with Scer\GAL4GMR85G01, Spyo\Cas9UAS.P2
decreased sleep | adult stage, with Scer\GAL4repo, Spyo\Cas9UAS.P2
decreased sleep | adult stage (with Df(3R)GBA1ΔTT)
long lived, with Scer\GAL4Act88F.1.3
short lived
short lived, with Scer\GAL4elav.PU
short lived | recessive
Phenotype manifest in
Allele
adult brain
adult brain, with Scer\GAL49-137, Scer\GAL4GMR83E12, Spyo\Cas9UAS.P2
adult brain, with Scer\GAL4GMR56F03, Scer\GAL4GMR85G01, Spyo\Cas9UAS.P2
adult brain, with Scer\GAL4nSyb.PU
adult brain, with Scer\GAL4repo
adult brain, with Scer\GAL4repo, Spyo\Cas9UAS.P2
adult brain (with Df(3R)GBA1ΔTT)
adult CNS glial cell
adult fat body
adult gnathal ganglion
adult gut
adult optic lobe, with Scer\GAL4GMR56F03, Scer\GAL4GMR85G01
adult s-LNv neuron | adult stage, with Gba1bΔ1/Df(3R)GBA1ΔTT, Scer\GAL4Pdf.PU
adult s-LNv neuron | adult stage, with Scer\GAL4GMR56F03, Scer\GAL4GMR85G01, Spyo\Cas9UAS.P2
adult s-LNv neuron | adult stage (with Df(3R)GBA1ΔTT)
adult s-LNv neuron | adult stage (with Df(3R)GBA1ΔTT), with Gba1b20xUAS.cVa, Scer\GAL4Pdf.PU
calyx of adult mushroom body (with Df(3R)GBA1ΔTT)
lysosome | adult stage
lysosome | adult stage, with Scer\GAL49-137, Scer\GAL4GMR83E12, Spyo\Cas9UAS.P2
lysosome | adult stage, with Scer\GAL4GMR56F03, Scer\GAL4GMR85G01
lysosome | adult stage, with Scer\GAL4GMR56F03, Scer\GAL4GMR85G01, Spyo\Cas9UAS.P2
lysosome | adult stage, with Scer\GAL4nSyb.PU
lysosome | adult stage, with Scer\GAL4repo
lysosome | adult stage, with Scer\GAL4repo, Spyo\Cas9UAS.P2
lysosome | adult stage (with Df(3R)GBA1ΔTT)
mitochondrion | adult stage | progressive
ommatidium
optic chiasma | adult stage (with Df(3R)GBA1ΔTT)
rhabdomere
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\GBA1
13 of 14
Yes
Yes
11  
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Gba
13 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Gba
12 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\gba
5 of 13
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\gba
13 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\gba-4
13 of 14
Yes
Yes
Cele\gba-3
11 of 14
No
Yes
Cele\gba-2
10 of 14
No
Yes
Cele\gba-1
9 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP001649
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Gba1b. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (1)
Gba1a
13 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 11 )
Potential Models Based on Orthology ( 7 )
Modifiers Based on Experimental Evidence ( 4 )
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
GBA1; glucosylceramidase beta 1
13 of 14
GLUCOSIDASE, BETA, ACID; GBA
 
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Interaction Browsers
Summary of Genetic Interactions
Interaction Browsers
View in FlyBase Interactions Browser   View in MIST tool (external link)
Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Gba1b
enhanceable
Gba1a
(Kinghorn et al., 2016)
Gba1b
enhanceable
per
(Vaughen et al., 2022)
Gba1b
enhanceable
Rel
(Atilano et al., 2023)
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
FlyBase
External Links
KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
External Data
Linkouts
KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Class of Gene
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3R
Recombination map
3-80
Cytogenetic map
95A9-95A10
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
95A9-95A10
Limits computationally determined from genome sequence between P{PZ}l(3)0690606906 and P{PZ}l(3)0468404684
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location

3-80

(FlyBase, 2016)

3-81.9

(Comeron et al., 2012)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (22)
Genomic Clones (18)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (4)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Vaughen et al., 2022)
      Commercially Available Antibodies
       
      Cell Line Information
      Publicly Available Cell Lines
       
        Other Stable Cell Lines
         
          Other Comments
          Relationship to Other Genes
          Source for database merge of
          Additional comments

          Annotation CG10299 split into CG31148, CG31413 and CG31414 in release 3 of the genome annotation.

          (FlyBase Genome Annotators, 2002-2003)
          Nomenclature History
          Source for database identify of

          Source for identity of: Gba1b CG31414

          (Suzuki et al., 2015)
          Nomenclature comments
          Etymology
          Synonyms and Secondary IDs (10)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          External Crossreferences and Linkouts ( 46 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          FlyMine - An integrated database for Drosophila genomics
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          Flygut - An atlas of the Drosophila adult midgut
          FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
          KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
          References (86)