GAL4 (and a Disc\RFPDsRed.3xP3.cUa marker) replaces approximately 437bp of endogenous trpl, near the translation initiation codon.
A 417bp region of trpl starting at the ATG has been replaced with GAL4 and a Disc\RFPDsRed.3xP3.cUa marker. The position of the deletion endpoints on the reference sequence was determined from sequence data and the primer sequences used to generate the homology arms.