FB2026_02 , released June 18, 2026
Allele: Scer\GAL4Spg7-CR70796-KO-TG4.0
Open Close
General Information
Symbol
Scer\GAL4Spg7-CR70796-KO-TG4.0
Species
S. cerevisiae
Name
FlyBase ID
FBal0390585
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
TI{CRIMIC.TG4.0}Spg7CR70796-KO-TG4.0
Carried in Construct
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Gene Disruption Project members, 2018-)
gene targeting by homologous recombination
(Gene Disruption Project members, 2018-)
Progenitor genotype
Associated Insertion(s)
TI{CRIMIC.TG4.0}Spg7CR70796-KO-TG4.0
Cytology
Description

A TI{CRIMIC.TG4.0} DNA cassette has been inserted into Spg7, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. In addition, the coding exons of Spg7 downstream of the inserted gene trap cassette have been deleted. The TI{CRIMIC.TG4.0} cassette was inserted via the CRISPR/Cas-9 hybrid technique, using two gRNAs that target Spg7 : one targeted to a coding intron (CAGCTGCATCACCTACAGAGAGG) and the other to a non-coding exon in the 3' UTR (TGAGTTGGACCCATGGCCTCAGG). The PCR check of the insertion gave no band for the left flank and the expected sized product for the right flank. The insertion junction of the right flank was verified by sequencing.

(Gene Disruption Project members, 2018-)
Allele components
Component
Use(s)
Inserted element
TI{CRIMIC.TG4.0}
Encoded product / tool
GAL4
Also carries
attP
FRT
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4Spg7-CR70796-KO-TG4.0
      Name Synonyms
      Secondary FlyBase IDs
        References (1)