A TI{KozakGAL4} DNA cassette has been inserted into Elp3, replacing the coding sequence (coordinates of deleted sequence are 2L:4444811..4446660 , release 6 genome). This results in a simultaneous knock-out of Elp3 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of Elp3 (predicted to gene trap all annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: GATATTCATCAGCAAAATGAAGG and ATAGCGTTAAATGACTGTCTTGG. The 3' end of the deletion associated with the insertion is 10 bp downstream of the 3' end of the MFS18 gene and may affect the proper 3' end formation of its transcripts.