A TI{KozakGAL4} DNA cassette has been inserted into Lamtor4, replacing the coding sequence (coordinates of deleted sequence are 3L:3896907..3897422 , release 6 genome). This results in a simultaneous knock-out of Lamtor4 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of Lamtor4 (predicted to gene trap all annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: TCCGGTCACACTGGTGGGTGTGG and ATGGGAGTGCGCCAATTGTGGGG. The 3' end of the deletion associated with the insertion is 22 bp downstream of the 3' end of the Ccz1 gene and may affect the proper 3' end formation of its transcripts.