A TI{KozakGAL4} DNA cassette has been inserted into Nup160, replacing the coding sequence (coordinates of deleted sequence are 2L:11123812..11128910 , release 6 genome). This results in a simultaneous knock-out of Nup160 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of Nup160 (predicted to gene trap all annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: ACTACTGAGCGTGTTTACGCCGG and GCAGATGCGAACGGATCTTCAGG. The 3' end of the deletion associated with the insertion is 22 bp downstream of the 3' end of the RfC38 gene and may affect the proper 3' end formation of its transcripts.