A TI{KozakGAL4} DNA cassette has been inserted into CG4593, replacing the coding sequence (coordinates of deleted sequence are X:7013914..7014617 , release 6 genome). This results in a simultaneous knock-out of CG4593 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of CG4593 (predicted to gene trap all annotated transcripts of the gene). The 5' end of the deletion associated with the insertion is 33 bp upstream of the 5' end of the CG3032 gene and may affect its transcription. The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: TTTCTTGGGAGGCTTGATTCAGG and CATGTAGTCCCCGGGTGGGCAGG.