A TI{CRIMIC.TG4.2} DNA cassette has been inserted into Paf1L, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The insertion is also within dpr3; it is in the wrong orientation to trap dpr3, but it is difficult to predict whether it might affect its expression. The TI{CRIMIC.TG4.2} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequence used to target the gene was: TTCCTGTCAATTCAAACTCGAGG.
