The GMR34C03 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
The GMR71D01 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
In a split-GAL4 system, HsapRELAAD.R34C03 drives expression in antennal grooming descending neuron 1, antennal grooming brain interneuron 1, the optic lobe and the thoracico-abdominal ganglion when combined with ScerGAL4DBD.R71D01 and in antennal grooming descending neuron 1 when combined with ScerGAL4DBD.R18C11.
In a split-GAL4 system, ScerGAL4DBD.R71D01 drives expression in antennal grooming descending neuron 1 when combined with HsapRELAAD.R18C11, in antennal grooming brain interneuron 1 and the adult central complex when combined with HsapRELAAD.R11B11, and in antennal grooming descending neuron 1, antennal grooming brain interneuron 1, the optic lobe and thoracico-abdominal ganglion when combined with HsapRELAAD.R34C03. Antennal grooming descending neuron 1 neurons were seen in close proximity to EcollexAGMR26B12 expressing antennal grooming brain interneuron 2 and EcollexAGMR27H08 expressing zone C/E Johnston organ neurons. Positive GRASP (GFP reconstitution across synaptic partners) signals were seen between C/E Johnston organ neurons and antennal grooming descending neuron 1 using these drivers.