FlyBase Gene Report: Dmel\Pig1

General Information

Symbol

Dmel\Pig1

Species

D. melanogaster

Name

Pre-intermoult gene 1

Annotation Symbol

CG10790

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003086

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Red Book
Interactive Fly

Also Known As

Pig-1, gsg

Key Links

Genomic Location

Cytogenetic map

3C10-3C10

Sequence location

X:3,248,600..3,249,289 [-]

Recombination map

1-2

RefSeq locus

NC_004354 REGION:3248600..3249289

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (0 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Pig1: Pre-intermolt gene 1

Expressed in larval salivary glands, RNA reaching a peak in second larval instar.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pig1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P26023)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Supported by strand-specific RNA-Seq data.

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pig1-RB

FBtr0070537

NM_057329

690

172

Additional Transcript Data and Comments

Reported size (kB)

0.69 (longest cDNA)

(Furia et al., 1991)

0.75 (northern blot)

(Chen et al., 1987)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Pig1-PB

FBpp0070512

18.5

172

10.36

P26023

NP_476677

AAF45859

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

183 (aa); 19.3 (kD predicted)

(Furia et al., 1991)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pig1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pig1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

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Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval salivary gland

(Chen et al., 1987)

organism

(Chen et al., 1987)

Additional Descriptive Data

Pig1 expression is detected in first through third larval instar, and peaks at second larval instar. Pig1 is detected in RNA isolated from salivary glands.

(Chen et al., 1987)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pig1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pig1

View exonic expression by tissue for Dmel\Pig1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31303/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003086

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003086

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003086

Flygut - An atlas of the Drosophila adult midgut

FBgn0003086

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pig1

Transgenic constructs containing regulatory region of Pig1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 21 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pig1^GD6400

viable, with Scer\GAL4^Mef2.PR

Pig1^GD6400

viable, with Scer\GAL4^pnr-MD237

Pig1^GD6400

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (1)

Xtro\ctr9

1 of 13

Yes

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pig1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pig1 - CG10209

two hybrid

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0003086

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-2

Cytogenetic map

3C10-3C10

Sequence location

X:3,248,600..3,249,289 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

3C10-3C10

Limits computationally determined from genome sequence between P{EP}dnc^EP1395 and P{EP}dnc^EP973

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

3C11-3C12

(determined by in situ hybridisation)

(Furia et al., 1991)

Experimentally Determined Recombination Data

Location

1-2

(FlyBase, 2016)

1-2.7

(Comeron et al., 2012)

Left of (cM)

Sgs4

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

54058

y¹ v¹; P{TRiP.HMJ21201}attP40

NIG-Fly

HMJ21201

y¹ v¹; P{TRiP.HMJ21201}attP40/CyO

HMJ22503

y¹ v¹; P{TRiP.HMJ22503}attP40/CyO

VDRC

v30981

w¹¹¹⁸; P{GD6400}v30981/TM3

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

RT02909

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003086

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003086

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The transcriptional switch between Pig1 and Sgs4 depends on a Sebp1 binding site within a shared enhancer region.

(Hofmann and Lehmann, 1998)

Pig1 RNA expression has been analysed in wild-type and br mutant larvae.

(D'Avino et al., 1995)

There is evidence that the br-C directly mediates the ecdysone-dependent transcriptional switch, that leads to Sgs4 induction and Pig1 repression, by binding to sites within element III.

(von Kalm et al., 1994)

Ecdysteroid-regulated gene.

(Andres et al., 1993)

Pre-intermoult gene expression in under the control of the br-C. Upstream sequences contain consensus sites for binding Eip74EF.

(Bornschein et al., 1993)

Three adjacent segments located within the short region between Sgs4 and Pig1 can account for the switch from Pig1 expression during late second to early third instar to Sgs4 expression at the end of third instar. Germline transformation studies reveal that a 170bp segment acts as an enhancer to direct Sgs4 expression, a 64bp sequence just upstream can modify the temporal specificity of the enhancer so it works throughout the third instar and just further upstream is a repressor element.

(Mougneau et al., 1993)

Pig1 was molecularly characterized by the use of cDNA isolation and sequencing. The gene is devoid of introns and can differentially use two alternative 3' and 5' ends.

(Furia et al., 1991)

The br wild type gene product, which is necessary for the activation of intermoult glue genes, is necessary for the inactivation of the pre-moult genes.

(Hofmann et al., 1991)

Pig1 and Sgs4 are located within the 79kb intron of the dnc gene. Pig1 is transcribed from the opposite strand to dnc and Sgs4.

(Chen et al., 1987)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CG10790

(National Institute of Genetics Fly Stocks, 2023-, Ni et al., 2010.12.1, Zhang et al., 2010, Whitfield, 2000.9.7)

EG:96G10.1

Pig1

(Hu et al., 2017.6.13, Lindsley and Zimm, 1992)

gsg

(Saumweber et al., 1990)

pig1

(Jensen et al., 2007, Li and White, 2003)

Name Synonyms

Pre-intermolt gene 1

(Lindsley and Zimm, 1992)

Pre-intermoult gene 1

gland specific gene

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 32 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31303

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P26023

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P26023

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q6BF93

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P26023

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31303/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003086

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003086

FlyMine - An integrated database for Drosophila genomics

FBgn0003086

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10790

MARRVEL_MODEL - MARRVEL (model organism gene)

31303

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003086

DroID - A comprehensive database of gene and protein interactions.

FBgn0003086

DRSC - Results frm RNAi screens

FBgn0003086

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pig1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003086

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003086

Flygut - An atlas of the Drosophila adult midgut

FBgn0003086

References (54)

The following external sites may use different assemblies or annotations than FlyBase.

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