FlyBase Gene Report: Dmel\Mst57Da

General Information

Symbol

Dmel\Mst57Da

Species

D. melanogaster

Name

Male-specific RNA 57Da

Annotation Symbol

CG9074

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011668

Gene Model Status

Current

Stock Availability

2 publicly available

Gene Summary

Male-specific RNA 57Da (Mst57Da) encodes a peptide that is produced by the male accessory glands and transferred to females during mating. Its proline-rich peptide composition suggests a possible role as an antimicrobial peptide. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

96F3-96F3

Sequence location

3R:25,831,575..25,831,899 [-]

Recombination map

3-89

RefSeq locus

NT_033777 REGION:25831575..25831899

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in insemination

inferred from high throughput expression pattern

(Findlay et al., 2009, Findlay et al., 2008)

involved_in mating

inferred from expression pattern

(Swanson et al., 2001)

inferred from high throughput expression pattern

(Mueller et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in egg-laying behavior

non-traceable author statement

(Simmerl et al., 1995)

involved_in regulation of female receptivity

non-traceable author statement

(Simmerl et al., 1995)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in extracellular space

inferred from high throughput direct assay

(Findlay et al., 2009, Findlay et al., 2008)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in extracellular region

non-traceable author statement

(Simmerl et al., 1995)

inferred from sequence model

(Swanson et al., 2001)

located_in extracellular space

inferred from sequence model

(Mueller et al., 2005)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Protein Function (UniProtKB)

Transferred from male to female during mating and may affect egglaying and behavior after mating.

(UniProt, Q9VBL6)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Mst57Da for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VBL6)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mst57Da-RA

FBtr0084914

NM_080167

325

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Mst57Da-PA

FBpp0084288

7.1

9.38

Q9VBL6

NP_524906

AAF56515

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VBL6

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mst57Da using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mst57Da_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

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Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular space

inferred from high throughput direct assay

(Findlay et al., 2009, Findlay et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mst57Da in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mst57Da

View exonic expression by tissue for Dmel\Mst57Da

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011668

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

47954/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011668

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011668

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011668

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9074

Flygut - An atlas of the Drosophila adult midgut

FBgn0011668

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mst57Da

Transgenic constructs containing regulatory region of Mst57Da

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Mst57Da^GD12933

viable, with Scer\GAL4^Mef2.PR

Mst57Da^GD12933

viable, with Scer\GAL4^pnr-MD237

Mst57Da^GD12933

viable, with Scer\GAL4^αTub84B.PL

Mst57Da^UAS.cMa

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Mst57Da. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

71071

DroID - A comprehensive database of gene and protein interactions.

FBgn0011668

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-89

Cytogenetic map

96F3-96F3

Sequence location

3R:25,831,575..25,831,899 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

96F3-96F3

Limits computationally determined from genome sequence between P{PZ}l(3)rQ197^rQ197 and P{lacW}scrib^j7B3

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

57D-57D

(determined by in situ hybridisation)

(Simmerl et al., 1995, Schafer, 1986)

Experimentally Determined Recombination Data

Location

3-89

(FlyBase, 2016)

3-86.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

23515

w¹¹¹⁸; Mi{ET1}Mst57Da^MB03151

VDRC

v40694

w¹¹¹⁸; P{GD12933}v40694

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (6)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH19893

Other clones

IP09740

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011668

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011668

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Isolated in a screen for seminal fluid proteins.

(Mueller et al., 2004)

The 5' untranslated regions of Mst57Da, Mst57Db and Mst57Dc are characterized by several short open reading frames. Mst57Da and Mst57Db share a conserved sequence block in front of the putative translated regions. These structural features may be important for control of these genes. Mst57Da and Mst57Db are secretory proteins and components of the paragonial secretions.

(Simmerl et al., 1995)

Isolated by differential screening of a genomic library with cDNA to poly(A)RNA from adult males, and poly(A)RNA from embryos and adult females.

(Schafer, 1986)

Mst57Da encodes a male-specific transcript.

(Schafer, 1986)

Relationship to Other Genes

Source for database merge of

Source for merge of: Mst57Da BcDNA:GH19893

(FlyBase, 1996-)

Additional comments

Source for merge of Mst57Da BcDNA:GH19893 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

BcDNA:GH19893

CG9074

(Mueller et al., 2007, Ram and Wolfner, 2007, Ravi Ram and Wolfner, 2007, Mueller et al., 2005, Mueller et al., 2004)

Mst57D

Mst57Da

(Duan et al., 2025, Cheng et al., 2024, Ghosh et al., 2020, Avila et al., 2015, Findlay et al., 2009, Fiumera et al., 2007, Ravi Ram and Wolfner, 2007, Reiter et al., 2007)

bs30d07.y1

(Andrews et al., 2000)

mst(2)ag-1

mst-1

(Schafer, 1986)

mst57D

(Kwon et al., 2009)

Name Synonyms

CG9074

(Ram and Wolfner, 2007)

Male-specific RNA 57Da

Secondary FlyBase IDs

FBgn0002859
FBgn0039377
FBgn0063235

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 30 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

47954

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VBL6

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VBL6

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VBL6

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011668

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

47954/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011668

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011668

FlyMine - An integrated database for Drosophila genomics

FBgn0011668

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9074

MARRVEL_MODEL - MARRVEL (model organism gene)

47954

Linkouts

BioGRID - A database of protein and genetic interactions.

71071

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011668

DroID - A comprehensive database of gene and protein interactions.

FBgn0011668

DRSC - Results frm RNAi screens

FBgn0011668

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mst57Da_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011668

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011668

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9074

Flygut - An atlas of the Drosophila adult midgut

FBgn0011668

References (50)

The following external sites may use different assemblies or annotations than FlyBase.

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