FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\mt:ATPase8
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General Information
Symbol
Dmel\mt:ATPase8
Species
D. melanogaster
Name
mitochondrial ATPase subunit 8
Annotation Symbol
CG34072
Feature Type
protein_coding_gene
FlyBase ID
FBgn0013673
Gene Model Status
Current
Stock Availability
1 publicly available
Gene Summary
mitochondrial ATPase subunit 8 (mt:ATPase8) is a mitochondrial DNA gene that encodes a subunit of the mitochondrial ATP synthase localized to the inner mitochondrial membrane. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

ATP8, ATPase8, mt:ATP8

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
RefSeq locus
NC_024511 REGION:3907..4068
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
Function
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables proton transmembrane transporter activity
inferred from electronic annotation with InterPro:IPR001421
(InterPro Project Members, 2004-)
contributes_to proton-transporting ATP synthase activity, rotational mechanism
non-traceable author statement
(Tripoli et al., 2005)
inferred from sequence or structural similarity with UniProtKB:P03928
(Gene Ontology Curators, 2002-)
Biological Process (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in proton motive force-driven ATP synthesis
inferred from electronic annotation with InterPro:IPR001421
(InterPro Project Members, 2004-)
involved_in proton motive force-driven mitochondrial ATP synthesis
inferred from sequence or structural similarity with UniProtKB:P03928
(Gene Ontology Curators, 2002-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in mitochondrial inner membrane
non-traceable author statement
(Tripoli et al., 2005)
inferred by curator from GO:0045259
(FlyBase Curators, 2016-)
part_of proton-transporting ATP synthase complex
inferred from sequence or structural similarity with UniProtKB:P03928
(Gene Ontology Curators, 2002-)
non-traceable author statement
(Ilinsky, 2013)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
Belongs to the ATPase protein 8 family. (P84345)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
proton-transporting ATP synthase activity, rotational mechanism
RHEA 57722:
Summaries
Gene Snapshot
mitochondrial ATPase subunit 8 (mt:ATPase8) is a mitochondrial DNA gene that encodes a subunit of the mitochondrial ATP synthase localized to the inner mitochondrial membrane. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
MITOCHONDRIAL COMPLEX V - F0 SUBUNITS -
Mitochondrial ATP synthase (Complex V) synthesizes ATP from ADP and inorganic phosphate using the energy provided by the proton electrochemical gradient across the inner mitochondrial membrane. It consists of two structural domains: the F1 sector, a soluble portion situated in the mitochondrial matrix, and the F0 sector, which spans the inner mitochondrial membrane. The F0 sector consists of an integral membrane proton-conducting channel and a peripheral stalk domain. (Adapted from PMID:21874297).
MITOCHONDRIAL COMPLEX V - TESTIS-SPECIFIC VARIANT -
Mitochondrial ATP synthase (Complex V) synthesizes ATP from ADP and inorganic phosphate using the energy provided by the proton electrochemical gradient (proton-motive force) across the inner mitochondrial membrane generated by electron transport complexes of the respiratory chain. It is an F-type ATPase and consists of two structural domains: the F1 sector, a soluble portion situated in the mitochondrial matrix, and the F0 sector, which spans the inner mitochondrial membrane. Protons pass from the inter membrane space to the matrix through F0, which transfers the energy created by the proton-motive force to F1 where ADP is phosphorylated to ATP using a rotational mechanism. (Adapted from PMID:23356252 and PMID:21874297).
MITOCHONDRIAL COMPLEX V -
Mitochondrial ATP synthase (Complex V) synthesizes ATP from ADP and inorganic phosphate using the energy provided by the proton electrochemical gradient (proton-motive force) across the inner mitochondrial membrane generated by electron transport complexes of the respiratory chain. It is an F-type ATPase and consists of two structural domains: the F1 sector, a soluble portion situated in the mitochondrial matrix, and the F0 sector, which spans the inner mitochondrial membrane. Protons pass from the inter membrane space to the matrix through F0, which transfers the energy created by the proton-motive force to F1 where ADP is phosphorylated to ATP using a rotational mechanism. (Adapted from PMID:23356252 and PMID:21874297).
Pathway (FlyBase)
OXIDATIVE PHOSPHORYLATION -
Oxidative phosphorylation (OXPHOS) is the coordinated transfer of electrons and protons leading to the production of ATP. The OXPHOS system is composed of five mitochondrial inner membrane complexes, Complexes I-IV comprise the electron respiratory chain and Complex V the ATP synthase. Complexes I and II oxidize the electron donors NADH and FADH2, respectively, derived from the tricarboxylic acid (TCA) cycle, and the electrons are transferred to the electron carrier ubiquinone which shuttles them to Complex III. Cytochrome c transfers electrons between Complex III and the terminal complex in the respiratory chain, Complex IV. Complex IV catalyzes electron transfer from reduced cytochrome c to oxygen. Complex I, II and IV couple electron transfer to proton transport across the inner mitochondrial membrane into the inner membrane space. This generates a proton electrochemical gradient which is used to drive the synthesis of ATP from ADP and inorganic phosphate by ATP synthase (Complex V). (Adapted from PMID:23356252 and PMID:11331900.)
Protein Function (UniProtKB)
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane (By similarity).
(UniProt, P84345)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\mt:ATPase8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry P84345)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
mt:ATPase8-RA
FBtr0100866
162
53
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
mt:ATPase8-PA
FBpp0100178
6.4
53
9.17
P84345
NP_008280
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel.

(UniProt, P84345)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mt:ATPase8 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mt:ATPase8 in JBrowse
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 0 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of mt:ATPase8
Transgenic constructs containing regulatory region of mt:ATPase8
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 0 )
    Variants
    Variant Molecular Consequences
    Alleles Representing Disease-Implicated Variants
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Phenotype manifest in
    Allele
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Human Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Homo sapiens (Human) (0)
    Model Organism Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Rattus norvegicus (Norway rat) (0)
    Mus musculus (laboratory mouse) (0)
    Xenopus tropicalis (Western clawed frog) (0)
    Danio rerio (Zebrafish) (0)
    Caenorhabditis elegans (Nematode, roundworm) (0)
    Anopheles gambiae (African malaria mosquito) (1)
    Agam\ATP8
    8 of 12
    Yes
    Yes
    Arabidopsis thaliana (thale-cress) (0)
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    Schizosaccharomyces pombe (Fission yeast) (0)
    Escherichia coli (enterobacterium) (0)
    Other Organism Orthologs (via OrthoDB)
    Data provided directly from OrthoDB:mt:ATPase8. Refer to their site for version information.
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v9.1)
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Interaction Browsers
      Summary of Genetic Interactions
      Interaction Browsers
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Subunit Structure (UniProtKB)
      F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel.
      (UniProt, P84345 )
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      OXIDATIVE PHOSPHORYLATION
      External Links
      KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
      External Data
      Linkouts
      KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      mitochondrion_genome
      Recombination map
      Cytogenetic map
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (1)
      Genomic Clones (0)
       
        cDNA Clones (0)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
          Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
                Other clones
                  RNAi and Array Information
                  Linkouts
                  DRSC - Results frm RNAi screens
                  Antibody Information
                  Laboratory Generated Antibodies
                   
                  Commercially Available Antibodies
                   
                  Cell Line Information
                  Publicly Available Cell Lines
                   
                    Other Stable Cell Lines
                     
                      Other Comments

                      New annotation (CG34072) in release 4.3 of the genome annotation.

                      (FlyBase Genome Annotators, 2005)
                      Relationship to Other Genes
                      Source for database merge of
                      Additional comments
                      Nomenclature History
                      Source for database identify of
                      Nomenclature comments
                      Etymology
                      Synonyms and Secondary IDs (10)
                      Datasets (0)
                      Study focus (0)
                      Experimental Role
                      Project
                      Project Type
                      Title
                      Study result (0)
                      Result
                      Result Type
                      Title
                      External Crossreferences and Linkouts ( 29 )
                      Sequence Crossreferences
                      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                      Other crossreferences
                      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                      FlyMine - An integrated database for Drosophila genomics
                      InterPro - A database of protein families, domains and functional sites
                      KEGG Genes - Molecular building blocks of life in the genomic space.
                      MARRVEL_MODEL - MARRVEL (model organism gene)
                      Linkouts
                      DroID - A comprehensive database of gene and protein interactions.
                      DRSC - Results frm RNAi screens
                      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                      KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                      KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                      References (41)