FlyBase Gene Report: Dmel\Acp76A

General Information

Symbol

Dmel\Acp76A

Species

D. melanogaster

Name

Accessory gland protein 76A

Annotation Symbol

CG3801

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015586

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Snapshot

Accessory gland protein 76A (Acp76A) encodes a protein that shows homology to the serpin serine endopeptidase inhibitor family but is predicted to be non-inhibitory. It is produced in the male accessory glands and transferred to females during mating. [Date last reviewed: 2019-03-07]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Spn76A, Accessory gland-specific peptide 76A

Key Links

Genomic Location

Cytogenetic map

75F5-75F5

Sequence location

3L:19,050,757..19,052,026 [-]

Recombination map

3-45

RefSeq locus

NT_037436 REGION:19050757..19052026

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables serine-type endopeptidase inhibitor activity

inferred from biological aspect of ancestor with PANTHER:PTN000156123

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from key residues

(Garrett et al., 2009)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in multicellular organism reproduction

inferred from high throughput expression pattern

(Findlay et al., 2009, Findlay et al., 2008, Mueller et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in negative regulation of endopeptidase activity

inferred from biological aspect of ancestor with PANTHER:PTN000156123

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in extracellular space

inferred from direct assay

(Coleman et al., 1995)

inferred from high throughput direct assay

(Findlay et al., 2009, Findlay et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000156123

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence model

(Mueller et al., 2005)

Gene Group (FlyBase)

NON-INHIBITORY SERPINS

Protein Family (UniProt)

Belongs to the serpin family. (Q9VVW1)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

NON-INHIBITORY SERPINS -: The serpin family is a structurally-related group of secreted proteins containing the serpin-fold, a 350-400 amino acid domain. The non-inhibitory serpins (SERine Protease INhibitors) lack the key proteinase cleavage site required to target proteases. The non-inhibitory serpins have diverse roles and in D.mel, many are uncharacterised. (Adapted from FBrf0213937 & FBrf0209216).

Protein Function (UniProtKB)

Responsible for physiological and behavioral changes in mated female flies. May play a role in accessory protein regulation and/or in the coagulation of seminal fluid to form a mating plug.

(UniProt, Q9VVW1)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Acp76A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.40

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Acp76A-RA

FBtr0075038

NM_079429

1201

388

Acp76A-RB

FBtr0308781

NM_001259902

1198

387

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Acp76A-PA

FBpp0074805

44.2

388

8.29

NP_524153

AAF49196

Acp76A-PB

FBpp0300938

44.1

387

8.55

NP_001246831

AFH04502

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

388 (aa)

(Wolfner et al., 1997)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Acp76A using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Acp76A_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

male accessory gland main cell

(Wolfner et al., 1997)

male accessory gland

(Wolfner et al., 1997)

Additional Descriptive Data

Expressed at high levels in male accessory gland main cells; not clear if transcript is expressed in secondary cells.

(Wolfner et al., 1997)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

female reproductive system

Comment: mated females

(Ravi Ram et al., 2005)

adult hemolymph

Comment: mated females

(Ravi Ram et al., 2005)

uterus

Comment: mated females

(Ravi Ram et al., 2005)

Additional Descriptive Data

Acp76A is transferred to the female at the time of mating and persists for about 2-3 hours in the female reproductive tract. Acp76A enters the female hemolymph. It is detected in the uterus.

(Ravi Ram et al., 2005)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular space

inferred from direct assay

(Coleman et al., 1995)

inferred from high throughput direct assay

(Findlay et al., 2009, Findlay et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Acp76A in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Acp76A

View exonic expression by tissue for Dmel\Acp76A

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015586

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3801-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0015586

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Acp76A

Transgenic constructs containing regulatory region of Acp76A

Deletions and Duplications ( 1 )

Disrupted in

Df(3L)nkd^ΔUpE

(Chang et al., 2008)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Acp76A^GD6178

viable, with Scer\GAL4^αTub84B.PL

Acp76A^UAS.cMa

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (24)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SERPINB12

2 of 15

Yes

Hsap\SERPINF2

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\SERPINH1

1 of 15

Hsap\SERPINI1

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (39)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Serpinf2

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Serpinh1

1 of 15

Mmus\Serpini1

1 of 15

Rattus norvegicus (Norway rat) (28)

Rnor\LOC100911107

1 of 13

Yes

Rnor\Serpina10

1 of 13

Yes

Rnor\Serpinb1a

1 of 13

Yes

Rnor\Serpinb1b

1 of 13

Yes

Rnor\Serpinb2

1 of 13

Yes

Rnor\Serpinb3a

1 of 13

Yes

Rnor\Serpinb3

1 of 13

Yes

Rnor\Serpinb5

1 of 13

Yes

Rnor\Serpinb6a

1 of 13

Yes

Rnor\Serpinb6b

1 of 13

Yes

Rnor\Serpinb7

1 of 13

Yes

Rnor\Serpinb8

1 of 13

Yes

Rnor\Serpinb9d

1 of 13

Yes

Rnor\Serpinb9

1 of 13

Yes

Rnor\Serpinb10

1 of 13

Yes

Rnor\Serpinb11

1 of 13

Yes

Rnor\Serpinb12

1 of 13

Yes

Rnor\Serpinb13

1 of 13

Yes

Rnor\Serpinc1

1 of 13

Yes

Rnor\Serpind1

1 of 13

Yes

Rnor\Serpine1

1 of 13

Yes

Rnor\Serpine2

1 of 13

Yes

Rnor\Serpine3

1 of 13

Yes

Rnor\Serpinf1

1 of 13

Yes

Rnor\Serpinf2

1 of 13

Yes

Rnor\Serping1

1 of 13

Yes

Rnor\Serpinh1

1 of 13

Yes

Rnor\Serpini1

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (9)

Xtro\serpinf2

2 of 12

Yes

Xtro\serpina10

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (22)

Drer\serpine1

2 of 15

Yes

Drer\serpinf2a

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\serpinf2b

1 of 15

Yes

Drer\serping1

1 of 15

Yes

Drer\serpinh1a

1 of 15

Drer\serpinh1b

1 of 15

Drer\serpinh2

1 of 15

Yes

Drer\serpini1

1 of 15

Drer\si:ch1073-416d2.3

1 of 15

Drer\zgc:173729

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\srp-1

2 of 15

Yes

Cele\srp-3

2 of 15

Yes

Cele\srp-6

2 of 15

Yes

Cele\srp-7

2 of 15

Yes

Cele\M01G12.2

1 of 15

Yes

Cele\srp-2

1 of 15

Cele\srp-8

1 of 15

Arabidopsis thaliana (thale-cress) (10)

Atha\AT1G51330

1 of 9

Yes

Atha\AT1G62160

1 of 9

Yes

Atha\AT1G62170

1 of 9

Yes

Atha\AT2G26390

1 of 9

Yes

Atha\AT2G35580

1 of 9

Yes

Atha\AT3G45220

1 of 9

Yes

Atha\CCP3

1 of 9

Yes

Atha\SERPIN1

1 of 9

Yes

Atha\SRP2

1 of 9

Yes

Atha\SRP3

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091909W5 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00011566

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila yakuba

Drosophila yakuba

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (29)

Gene Symbol

Score

Source

Alignment

1 of 10

nec

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SERPINF2; serpin family F member 2

model of alpha-2-plasmin inhibitor deficiency

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0015586

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-45

Cytogenetic map

75F5-75F5

Sequence location

3L:19,050,757..19,052,026 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

75F5-75F5

Limits computationally determined from genome sequence between P{lacW}l(3)j4E6^j4E6 and P{EP}MESR6^EP3142

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

76A-76A

(determined by in situ hybridisation)

(Wolfner et al., 1997, Clark et al., 1995, Coleman et al., 1995)

Experimentally Determined Recombination Data

Location

3-45

(FlyBase, 2016)

3-42.3

(Comeron et al., 2012)

(Coleman et al., 1995)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Bloomington

33478

y¹ w^*; Mi{MIC}Acp76A^MI02019

65182

y¹ sc^* v¹ sev²¹; P{TRiP.HMC06056}attP40

VDRC

v108698

P{KK105959}VIE-260B

v51600

w¹¹¹⁸; P{GD6178}v51600/CyO

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (2)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP02514

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015586

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

IP02513

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015586

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40078

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

Protein contains putative non-inhibitory serpin fold.

(Gubb, 2012.9.27)

Isolated in a screen for seminal fluid proteins.

(Mueller et al., 2004)

Gene is identified in a differential cDNA hybridisation screen.

(Wolfner et al., 1997)

Encodes a male accessory-gland polypeptide. Expressed in males, but not in females. Protein level drops dramatically on mating, but recovers within 24 hours.

(Coleman et al., 1995)

Acp76A protein synthesised in the male accessory gland is transferred to females during mating.

(Coleman et al., 1995)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 49 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40078

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VVW1

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9NEE3

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015586

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Acp76A_1

Flygut - An atlas of the Drosophila adult midgut

FBgn0015586

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40078

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG3801

modMine - A data warehouse for the modENCODE project

FBgn0015586

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0015586

DRSC - Results frm RNAi screens

FBgn0015586

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015586

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3801-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015586

FlyMine - An integrated database for Drosophila genomics

FBgn0015586

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Name Synonyms

Accessory gland protein 76A

(Wolfner et al., 1997)

Accessory gland-specific peptide 76A

(Hashimoto et al., 2012.10.8, Takemori and Yamamoto, 2009, Coleman et al., 1995)

CG3801

(Ram and Wolfner, 2007)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (60)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections