FlyBase Gene Report: Dmel\Spn42Da

General Information

Symbol

Dmel\Spn42Da

Species

D. melanogaster

Name

Serpin 42Da

Annotation Symbol

CG9453

Feature Type

protein_coding_gene

FlyBase ID

FBgn0265137

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

Serpin 42Da (Spn42Da) encodes an endopeptidase inhibitor involved in hormone processing regulation. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Spn4, sp4, Serine protease inhibitor 4, Spn4A, serpin 4

Key Links

Genomic Location

Cytogenetic map

42D6-42D6

Sequence location

2R:6,875,906..6,878,927 [-]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:6875906..6878927

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables cysteine-type endopeptidase inhibitor activity

inferred from direct assay

(Bruning et al., 2007)

enables serine-type endopeptidase inhibitor activity

inferred from direct assay

(Bruning et al., 2007, Osterwalder et al., 2004, Richer et al., 2004)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables serine-type endopeptidase inhibitor activity

inferred from electronic annotation with InterPro:IPR000215

(InterPro Project Members, 2004-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in negative regulation of peptide hormone processing

inferred from mutant phenotype

(Osterwalder et al., 2004)

involved_in negative regulation of protein processing

inferred from mutant phenotype

(Osterwalder et al., 2004)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of immune response

inferred from biological aspect of ancestor with PANTHER:PTN000894695

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in extracellular space

inferred from direct assay

(Bruning et al., 2007)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000156123

(GO Reference Genome Project, 2011-)

located_in extracellular space

inferred from electronic annotation with InterPro:IPR000215

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

INHIBITORY SERPINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Serpin 42Da (Spn42Da) encodes an endopeptidase inhibitor involved in hormone processing regulation. [Date last reviewed: 2019-07-11]

Gene Group (FlyBase)

INHIBITORY SERPINS -: The serpin family is a structurally-related group of secreted proteins containing the serpin-fold, a 350-400 amino acid domain. Serpins (SERine Protease INhibitors) were first identified as a set of proteins able to inhibit proteases, acting by forming a covalent complex is formed with the target proteinase targeting it for degradation. (Adapted from FBrf0213937 and FBrf0209216).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Spn42Da for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7K8Y5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

4P0F
4P0O

Comments on Gene Model

Gene model reviewed during 5.48

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Spn42Da-RA

FBtr0086197

NM_165496

1505

392

Spn42Da-RB

FBtr0086196

NM_080216

1385

424

Spn42Da-RD

FBtr0086198

NM_206039

1531

411

Spn42Da-RE

FBtr0086199

NM_206038

1643

411

Spn42Da-RF

FBtr0086200

NM_206037

1669

411

Spn42Da-RG

FBtr0086201

NM_206036

1651

379

Spn42Da-RH

FBtr0086202

NM_206035

1763

379

Spn42Da-RI

FBtr0086203

NM_206034

1370

406

Spn42Da-RJ

FBtr0086204

NM_206033

1490

374

Spn42Da-RK

FBtr0086205

NM_206032

1789

379

Spn42Da-RL

FBtr0300336

NM_165495

2155

379

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Spn42Da-PA

FBpp0085524

44.1

392

5.94

FBpp0085523

47.7

424

5.91

FBpp0089189

46.0

411

6.59

FBpp0089190

46.2

411

6.51

FBpp0089191

46.2

411

6.51

FBpp0089192

42.4

379

6.69

FBpp0089193

42.7

379

6.59

FBpp0089207

45.6

406

7.01

FBpp0089208

42.1

374

7.18

FBpp0089206

42.7

379

6.59

FBpp0289565

42.7

379

6.59

Q8MM39

NP_724511

AAF57405

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

411 aa isoforms: Spn42Da-PE, Spn42Da-PF

379 aa isoforms: Spn42Da-PH, Spn42Da-PK, Spn42Da-PL

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

4P0F
4P0O

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Spn42Da using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.67

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

dorsal ectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

amnioserosa

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

ventral epidermis primordium

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular space

inferred from direct assay

(Bruning et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Spn42Da in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Spn42Da

View exonic expression by tissue for Dmel\Spn42Da

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0265137

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

49805/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0265137

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028985

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0265137

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9453

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Spn42Da

Transgenic constructs containing regulatory region of Spn42Da

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel6051

(Ryder, 2004.4.26)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

Spn42Da^GD3481

lethal | larval stage, with Scer\GAL4^dimm-929

Spn42Da^4.1.UAS

lethal | pupal stage, with Scer\GAL4^dimm-929

Spn42Da^4.1.UAS

lethal - all die before end of larval stage, with Scer\GAL4^how-24B

Spn42Da^4.1.UAS

partially lethal, with Scer\GAL4^tin.CΔ4

Spn42Da^GD3481

viable

Spn42Da^d08261

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Spn42Da^GD3481

viable, with Scer\GAL4^Mef2.PR

Spn42Da^GD3481

Sterility

Allele

fertile

Spn42Da^d08261

Other Phenotypes

Allele

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Spn42Da^GD3481

some die during larval stage, with Scer\GAL4^how-24B

Spn42Da^4.1.UAS

Phenotype manifest in

Allele

cephalopharyngeal skeleton, with Scer\GAL4^dimm-929

Spn42Da^4.1.UAS

cephalopharyngeal skeleton, with Scer\GAL4^how-24B

Spn42Da^4.1.UAS

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (38)

Hsap\SERPINI1

12 of 14

Yes

10 of 14

9 of 14

9 of 14

9 of 14

Yes

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

7 of 14

7 of 14

6 of 14

5 of 14

5 of 14

5 of 14

Yes

Hsap\HMSD

4 of 14

Yes

Hsap\SERPINA4

4 of 14

Yes

Hsap\SERPINA9

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Hsap\SERPINE1

3 of 14

Yes

Hsap\SERPINE3

3 of 14

Yes

Hsap\SERPINA13P

2 of 14

Yes

Hsap\SERPINF1

2 of 14

Yes

Hsap\SERPINF2

2 of 14

Yes

Hsap\SERPING1

2 of 14

Yes

Hsap\SERPINH1

2 of 14

Hsap\AGT

1 of 14

Hsap\SERPINA10

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (47)

Rnor\Serpini1

12 of 14

Yes

10 of 14

9 of 14

9 of 14

9 of 14

9 of 14

9 of 14

Yes

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

7 of 14

7 of 14

7 of 14

6 of 14

5 of 14

5 of 14

5 of 14

Yes

Rnor\Serpina4

4 of 14

Yes

Rnor\Serpina9

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Rnor\Serpina7

3 of 14

Yes

Rnor\Serpina11

3 of 14

Yes

Rnor\Serpina12

3 of 14

Rnor\Serpind1

3 of 14

Yes

Rnor\Serpine1

3 of 14

Yes

Rnor\Serpine3

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Rnor\Serpinf2

2 of 14

Yes

Rnor\Serping1

2 of 14

Yes

Rnor\RGD1564786

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Serpinh1

1 of 14

Mus musculus (laboratory mouse) (63)

Mmus\Serpini1

12 of 14

Yes

10 of 14

10 of 14

10 of 14

9 of 14

9 of 14

9 of 14

9 of 14

9 of 14

9 of 14

9 of 14

Yes

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

7 of 14

7 of 14

7 of 14

7 of 14

7 of 14

6 of 14

5 of 14

5 of 14

5 of 14

Yes

Mmus\Serpina3g

4 of 14

Yes

Mmus\Serpina9

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Mmus\Serpina3k

3 of 14

Mmus\Serpina3m

3 of 14

Yes

Mmus\Serpina3n

3 of 14

Mmus\Serpina5

3 of 14

Mmus\Serpina6

3 of 14

Yes

Mmus\Serpina12

3 of 14

Mmus\Serpind1

3 of 14

Yes

Mmus\Serpine1

3 of 14

Yes

Mmus\Serpine3

3 of 14

Yes

Mmus\Serpina7

2 of 14

Mmus\Serpina11

2 of 14

Mmus\Serpinf1

2 of 14

Yes

Mmus\Serpinf2

2 of 14

Yes

Mmus\Serping1

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (25)

Xtro\serpinb1

8 of 13

Yes

Xtro\serpinb4

7 of 13

Xtro\serpinb6

7 of 13

Xtro\serpini1

7 of 13

Yes

6 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

Yes

Xtro\serpina1

3 of 13

Xtro\serpinc1

3 of 13

Xtro\serpine3

3 of 13

Yes

Xtro\serpina3m

2 of 13

Xtro\serpine1

2 of 13

Yes

Xtro\serpine2

2 of 13

Yes

Xtro\serpinf1

2 of 13

Yes

Xtro\serpinf2

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\serpinh1

1 of 13

Yes

Danio rerio (Zebrafish) (30)

Drer\serpinb1

10 of 14

Yes

Drer\serpinb1l1

10 of 14

Yes

Drer\serpinb1l2

10 of 14

Yes

Drer\serpinb1l3

10 of 14

Yes

Drer\serpinb1l4

10 of 14

Yes

Drer\serpini1

10 of 14

Yes

Drer\zgc:173729

10 of 14

Yes

Drer\serpinb14

9 of 14

Yes

Drer\serpinc1

8 of 14

Yes

Drer\serpine2

5 of 14

Yes

Drer\LOC110439158

3 of 14

Drer\serpind1

3 of 14

Yes

Drer\serpine1

3 of 14

Yes

Drer\serpine3

3 of 14

Yes

Drer\si:ch211-186e20.7

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\serpinf2a

2 of 14

Yes

Drer\serpinf2b

2 of 14

Yes

Drer\serping1

2 of 14

Yes

Drer\serpinh1b

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Drer\serpina10a

1 of 14

Yes

Drer\serpina10b

1 of 14

Drer\serpinh1a

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (10)

Cele\srp-2

12 of 14

Yes

Cele\srp-3

12 of 14

Yes

Cele\srp-6

12 of 14

Yes

11 of 14

11 of 14

10 of 14

1 of 14

Yes

Cele\F44G3.7

1 of 14

Yes

Cele\srp-5

1 of 14

Cele\T01D3.3

1 of 14

Anopheles gambiae (African malaria mosquito) (18)

Agam\SRPN10

11 of 12

Yes

Agam\SRPN1

5 of 12

Agam\SRPN2

5 of 12

Agam\SRPN11

5 of 12

Yes

Agam\SRPN3

4 of 12

Agam\SRPN8

4 of 12

Agam\SRPN17

4 of 12

Yes

Agam\AgaP_AGAP012938

3 of 12

Agam\SRPN7

3 of 12

Yes

Agam\SRPN15

3 of 12

Agam\SRPN16

3 of 12

Agam\SRPN18

3 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

Yes

Agam\SRPN12

1 of 12

Agam\SRPN19

1 of 12

Arabidopsis thaliana (thale-cress) (13)

Atha\AT2G26390

9 of 13

Yes

Atha\AT3G45220

9 of 13

Yes

Atha\CCP3

9 of 13

Yes

Atha\SERPIN1

9 of 13

Yes

8 of 13

8 of 13

8 of 13

8 of 13

5 of 13

3 of 13

1 of 13

Yes

Atha\AT1G63280

1 of 13

Yes

Atha\AT2G35555

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Spn42Da. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (29)

12 of 13

11 of 13

11 of 13

11 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

nec

9 of 13

9 of 13

8 of 13

8 of 13

6 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

4 of 13

3 of 13

3 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

SERPINI1; serpin family I member 1

model of familial encephalopathy with neuroserpin inclusion bodies

IEA

(FlyBase, 2019-)

SERPINA6; serpin family A member 6

model of corticosteroid-binding globulin deficiency

IEA

(FlyBase, 2019-)

SERPIND1; serpin family D member 1

model of heparin cofactor II deficiency

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Spn42Da^KK107708

exacerbates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cJa

(Moore et al., 2025)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SERPINI1; serpin family I member 1

12 of 14

SERINE PROTEASE INHIBITOR, CLADE I, MEMBER 1; SERPINI1

ENCEPHALOPATHY, FAMILIAL, WITH NEUROSERPIN INCLUSION BODIES; FENIB

familial encephalopathy with neuroserpin inclusion bodies

SERPINB1; serpin family B member 1

10 of 14

130135

SERPINB3; serpin family B member 3

9 of 14

600517

SERPINB6; serpin family B member 6

9 of 14

173321

DEAFNESS, AUTOSOMAL RECESSIVE 91; DFNB91

autosomal recessive nonsyndromic deafness 91

SERPINI2; serpin family I member 2

9 of 14

605587

SERPINB4; serpin family B member 4

8 of 14

600518

SERPINB9; serpin family B member 9

8 of 14

601799

SERPINB10; serpin family B member 10

8 of 14

602058

SERPINB11; serpin family B member 11

8 of 14

615682

SERPINB12; serpin family B member 12

8 of 14

615662

SERPINB13; serpin family B member 13

8 of 14

604445

SERPINB2; serpin family B member 2

7 of 14

173390

SERPINB8; serpin family B member 8

7 of 14

601697

PEELING SKIN SYNDROME 5; PSS5

peeling skin syndrome 5

SERPINC1; serpin family C member 1

6 of 14

107300

ANTITHROMBIN III DEFICIENCY; AT3D

antithrombin III deficiency

SERPINB5; serpin family B member 5

5 of 14

154790

SERPINB7; serpin family B member 7

5 of 14

603357

PALMOPLANTAR KERATODERMA, NAGASHIMA TYPE; PPKN

Nagashima-type palmoplantar keratosis

SERPINE2; serpin family E member 2

5 of 14

177010

HMSD; histocompatibility minor serpin domain containing

4 of 14

612086

SERPINA4; serpin family A member 4

4 of 14

147935

SERPINA9; serpin family A member 9

4 of 14

615677

SERPINA1; serpin family A member 1

3 of 14

SERPIN PEPTIDASE INHIBITOR, CLADE A, MEMBER 1; SERPINA1

ALPHA-1-ANTITRYPSIN DEFICIENCY; A1ATD

alpha 1-antitrypsin deficiency

SERPINA2; serpin family A member 2 (gene/pseudogene)

3 of 14

107410

SERPINA3; serpin family A member 3

3 of 14

107280

SERPINA5; serpin family A member 5

3 of 14

601841

SERPINA6; serpin family A member 6

3 of 14

122500

CORTICOSTEROID-BINDING GLOBULIN DEFICIENCY

corticosteroid-binding globulin deficiency

SERPINA7; serpin family A member 7

3 of 14

314200

THYROXINE-BINDING GLOBULIN QUANTITATIVE TRAIT LOCUS; TBGQTL

SERPINA11; serpin family A member 11

3 of 14

619619

SERPINA12; serpin family A member 12

3 of 14

617471

SERPIND1; serpin family D member 1

3 of 14

142360

HEPARIN COFACTOR II DEFICIENCY

heparin cofactor II deficiency

SERPINE1; serpin family E member 1

3 of 14

173360

PLASMINOGEN ACTIVATOR INHIBITOR-1 DEFICIENCY

SERPINE3; serpin family E member 3

3 of 14

620989

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Spn42Da - Nrd1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Yoon et al., 2017)

Spn42Da - amon

comigration in sds page, western blot

(Richer et al., 2004)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

norpA

suppressible

Spn42Da

(Kinser and Dolph, 2012)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0265137

MIST (protein-protein) - An integrated Molecular Interaction Database

49805

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Glucocorticoid biosynthesis

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42D6-42D6

Sequence location

2R:6,875,906..6,878,927 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42D6-42D6

Limits computationally determined from genome sequence between P{lacW}ubl^k03203&P{PZ}CG3287⁰⁹⁹⁶⁷ and P{EP}EP2303&P{EP}EP2105

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

42D1-42D5

(determined by in situ hybridisation)

(Han et al., 2000)

42D-42E

(determined by in situ hybridisation)

(Osterwalder et al., 1999)

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-56.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

19291

w¹¹¹⁸; P{XP}Spn42Da^d08261

41613

y¹ v¹; P{TRiP.GL01195}attP40

31979

y¹ v¹; P{TRiP.JF03413}attP2/TM3, Sb¹

41722

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02288}attP2

NIG-Fly

2RG-1553

y² VhaAC39-1^cho-1 v¹; P{U6-Spn42Da.NIG.sgRNA}attP40/CyO

VDRC

v106306

P{KK107708}VIE-260B

v40973

w¹¹¹⁸; P{GD3481}v40973

v318090

PBac{fTRG00430.sfGFP-TVPTBF}VK00033

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (85)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0265137

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Osterwalder et al., 2004)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Overexpression of Spn4 either in the mesoderm or in peptidergic neurons results in animals which develop normally to the end of the first larval instar, but are unable to moult.

(Osterwalder et al., 2002)

Identification: Identified on the basis of sequence similarity to serpins.

(Osterwalder et al., 1999)

Relationship to Other Genes

Source for database merge of

Source for merge of: sp4 CG9453

(Bayraktaroglu, 2000.8.7)

Source for merge of: Spn4 Serp2

(FlyBase, 1992-)

Additional comments

'Spn4' and 'Serp2' reports merged because: 'dSerp2' was mentioned in a single reference (FBrf0107050) as being similar to vertebrate 'neuroserpin' and that it maps to 42D-E. A later reference from the same author (FBrf0146814) says that 'neuroserpin' corresponds to CG9453/Spn4, a gene that has been mapped experimentally to 42D.

(FlyBase, 1992-)

Nomenclature History

Source for database identify of

Source for identity of: Spn4 neuroserpin

(Osterwalder, 2002.3.25)

Source for identity of: Spn42Da Spn4

(Hashimoto et al., 2012.10.8)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG9453

(National Institute of Genetics Fly Stocks, 2022-, Kim et al., 2021, Hosono et al., 2015, Cammarato et al., 2011, Ni et al., 2010.12.1, Garrett et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Richer et al., 2004, De Gregorio et al., 2001)

Serp2

Sp4

(Christophides et al., 2002, De Gregorio et al., 2001)

Spn4

(Sarov et al., 2016, Sfaxi et al., 2014, Gendrin et al., 2013, Handke et al., 2013, Short and Lazzaro, 2013, Stefanatos et al., 2013, Garelli et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Ahmad et al., 2009, Buchon et al., 2009, Bruning et al., 2007, Kim et al., 2007, Osterwalder et al., 2004, Hashimoto et al., 2003)

Spn42Da

(Moore et al., 2025, Bossen et al., 2023, Pavlidaki et al., 2022, Wu et al., 2022, Jeon et al., 2020, Yoon et al., 2017, Hashimoto et al., 2012.10.8, Reichhart et al., 2011, Garrett et al., 2009)

Spn4A

(Meekins et al., 2017, Sfaxi et al., 2014, Richer et al., 2004)

dSerp2

(Osterwalder et al., 1999)

sp4

(van Steensel et al., 2003, Kruger et al., 2002, Han et al., 2000)

spn4

(Börner and Ragg, 2008)

Name Synonyms

Serine protease inhibitor 4

(Cammarato et al., 2011, Osterwalder et al., 2004)

Serp2

Serpin 4

(Kinser and Dolph, 2012)

Serpin 42 Da

(Meekins et al., 2017)

Serpin 42Da

(Hashimoto et al., 2012.10.8)

neuroserpin

(Osterwalder et al., 2002)

serpin 4

(Kinser and Dolph, 2012, Han et al., 2000)

Secondary FlyBase IDs

FBgn0028985
FBgn0033111
FBgn0026360

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 118 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

49805

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7K8Y5

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7K8Y5

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0265137

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

49805/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0265137

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0265137

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9453

MARRVEL_MODEL - MARRVEL (model organism gene)

49805

PDB - An information portal to biological macromolecular structures

4P0F
4P0O

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0265137

DroID - A comprehensive database of gene and protein interactions.

FBgn0265137

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028985

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0265137

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9453

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0265137

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

MIST (protein-protein) - An integrated Molecular Interaction Database

49805

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Glucocorticoid biosynthesis

References (82)

The following external sites may use different assemblies or annotations than FlyBase.

-0.67

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