FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Acp62F
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General Information
Symbol
Dmel\Acp62F
Species
D. melanogaster
Name
Accessory gland protein 62F
Annotation Symbol
CG1262
Feature Type
protein_coding_gene
FlyBase ID
FBgn0020509
Gene Model Status
Current
Stock Availability
29 publicly available
Gene Summary
Accessory gland protein 62F (Acp62F) encodes a trypsin inhibitor that is synthesized in male accessory glands and transferred to females during mating. It influences sperm storage in mated females. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Accessory gland peptide 62F

Key Links
Genomic Location
Cytogenetic map
62F2-62F2
Sequence location
Recombination map
3-4
RefSeq locus
NT_037436 REGION:2739750..2740228
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables serine-type endopeptidase inhibitor activity
inferred from direct assay
(Lung et al., 2002)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in insemination
inferred from high throughput expression pattern
(Findlay et al., 2009, Findlay et al., 2008)
involved_in mating
inferred from high throughput expression pattern
(Mueller et al., 2005)
involved_in negative regulation of proteolysis
inferred from mutant phenotype
(Mueller et al., 2008)
inferred from direct assay
(Lung et al., 2002)
involved_in sperm competition
inferred from mutant phenotype
(Mueller et al., 2008)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in extracellular space
inferred from high throughput direct assay
(Findlay et al., 2009, Findlay et al., 2008)
inferred from direct assay
(Lung et al., 2002)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Accessory gland protein 62F (Acp62F) encodes a trypsin inhibitor that is synthesized in male accessory glands and transferred to females during mating. It influences sperm storage in mated females. [Date last reviewed: 2019-03-07]
Protein Function (UniProtKB)
Responsible for physiological and behavioral changes in mated female flies. May contribute to the toxicity of seminal fluid and the decreased life-span of mated females. May also affect neuromuscular events after mating concerning sperm storage and egg release.
(UniProt, O46202)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Acp62F for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry O46202)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Acp62F-RA
FBtr0072943
NM_079168
479
115
Additional Transcript Data and Comments
Reported size (kB)

0.45 (northern blot)

(Wolfner et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Acp62F-PA
FBpp0072820
12.6
115
7.36
O46202
NP_523892
AAF47683
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

115 (aa)

(Wolfner et al., 1997)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Acp62F using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.27

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
female reproductive system

Comment: mated females

(Ravi Ram et al., 2005)
adult hemolymph

Comment: mated females

(Ravi Ram et al., 2005)
uterus

Comment: mated females

(Ravi Ram et al., 2005)
spermatheca

Comment: mated females

(Ravi Ram et al., 2005)
seminal receptacle

Comment: mated females

(Ravi Ram et al., 2005)
oviduct

Comment: mated females

(Ravi Ram et al., 2005)
ovary

Comment: mated females

(Ravi Ram et al., 2005)
oocyte

Comment: mated females

(Ravi Ram et al., 2005)
egg

Comment: mated females

(Ravi Ram et al., 2005)
Additional Descriptive Data

Acp62F is transferred to the female at the time of mating and persists for about 2-3 hours in the female reproductive tract. Acp62F enters the female hemolymph. It is detected in the uterus, sperm mass, spermathecum, seminal receptacle, oviduct, ovary, mature oocyte, and eggs.

(Ravi Ram et al., 2005)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in extracellular space
inferred from high throughput direct assay
(Findlay et al., 2009, Findlay et al., 2008)
inferred from direct assay
(Lung et al., 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Acp62F in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Acp62F
Transgenic constructs containing regulatory region of Acp62F
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3L)Exel6091
(Ryder, 2004.4.26)
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4αTub84B.PL
lethal | heat sensitive, with Scer\GAL4hs.PB
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (6)
Hsap\FCGBP
1 of 14
Yes
No
Hsap\MUC5B
1 of 14
Yes
No
Hsap\MUC6
1 of 14
Yes
No
Hsap\MUC19
1 of 14
Yes
No
Hsap\VWF
1 of 14
Yes
No
Hsap\ZAN
1 of 14
Yes
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Muc5ac
1 of 14
Yes
No
Rnor\Muc6
1 of 14
Yes
No
Mus musculus (laboratory mouse) (4)
Mmus\Muc5ac
1 of 14
Yes
No
Mmus\Muc6
1 of 14
Yes
No
Mmus\Muc19
1 of 14
Yes
No
Mmus\Vwf
1 of 14
Yes
No
Xenopus tropicalis (Western clawed frog) (10)
Xtro\LOC100487636
1 of 13
Yes
Yes
Xtro\LOC100487808
1 of 13
Yes
No
Xtro\LOC100487969
1 of 13
Yes
No
Xtro\LOC100493186
1 of 13
Yes
No
Xtro\LOC100494280
1 of 13
Yes
Yes
Xtro\LOC101733006
1 of 13
Yes
Yes
Xtro\LOC101733954
1 of 13
Yes
No
Xtro\LOC101734536
1 of 13
Yes
Yes
Xtro\LOC108648061
1 of 13
Yes
Yes
Xtro\vwf
1 of 13
Yes
No
Danio rerio (Zebrafish) (4)
Drer\hmcn2
1 of 14
Yes
No
Drer\muc5.2
1 of 14
Yes
No
Drer\sspo
1 of 14
Yes
No
Drer\tecta
1 of 14
Yes
No
Caenorhabditis elegans (Nematode, roundworm) (16)
Cele\F53C11.9
3 of 14
Yes
Yes
Cele\C10G8.4
2 of 14
No
No
Cele\C25E10.8
2 of 14
No
No
Cele\swm-1
2 of 14
No
No
Cele\Y69H2.3
2 of 14
No
No
Cele\Y69H2.10
2 of 14
No
No
Cele\B0238.12
1 of 14
No
No
Cele\C25E10.7
1 of 14
No
No
Cele\C25E10.10
1 of 14
No
No
Cele\C53B7.2
1 of 14
No
Yes
Cele\F32D8.3
1 of 14
No
No
Cele\F36H9.4
1 of 14
No
No
Cele\F47G3.4
1 of 14
No
Yes
Cele\spi-1
1 of 14
No
No
Cele\T06E6.10
1 of 14
No
No
Cele\Y26D4A.12
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (25)
Agam\AgaP_AGAP001176
2 of 12
Yes
Yes
Agam\AgaP_AGAP002445
2 of 12
Yes
No
Agam\AgaP_AGAP002446
2 of 12
Yes
No
Agam\AgaP_AGAP002641
2 of 12
Yes
No
Agam\AgaP_AGAP006252
2 of 12
Yes
No
Agam\AgaP_AGAP006253
2 of 12
Yes
No
Agam\AgaP_AGAP006581
2 of 12
Yes
No
Agam\AgaP_AGAP006582
2 of 12
Yes
No
Agam\AgaP_AGAP006584
2 of 12
Yes
No
Agam\AgaP_AGAP006585
2 of 12
Yes
No
Agam\AgaP_AGAP006586
2 of 12
Yes
No
Agam\AgaP_AGAP006587
2 of 12
Yes
No
Agam\AgaP_AGAP006589
2 of 12
Yes
No
Agam\AgaP_AGAP006813
2 of 12
Yes
No
Agam\AgaP_AGAP012970
2 of 12
Yes
No
Agam\AgaP_AGAP002447
1 of 12
No
No
Agam\AgaP_AGAP002448
1 of 12
No
No
Agam\AgaP_AGAP002636
1 of 12
No
Yes
Agam\AgaP_AGAP006523
1 of 12
No
No
Agam\AgaP_AGAP006524
1 of 12
No
Yes
Agam\AgaP_AGAP006525
1 of 12
No
Yes
Agam\AgaP_AGAP006527
1 of 12
No
No
Agam\AgaP_AGAP006583
1 of 12
No
No
Agam\AgaP_AGAP006588
1 of 12
No
No
Agam\AgaP_AGAP013045
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Acp62F. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (10)
CG33259
5 of 13
CG5267
3 of 13
CG34189
3 of 13
CadN2
1 of 13
CG42259
1 of 13
CG42878
1 of 13
CG43229
1 of 13
CG45546
1 of 13
Lrp4
1 of 13
Sfp53D
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Interaction Browsers
    Summary of Genetic Interactions
    Interaction Browsers
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map
    3-4
    Cytogenetic map
    62F2-62F2
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    62F2-62F2
    Limits computationally determined from genome sequence between P{PZ}Spn06911 and P{PZ}l(3)0680306803
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    62F-62F
    (determined by in situ hybridisation)
    (Wolfner et al., 1997)
    Experimentally Determined Recombination Data
    Location

    3-4

    (FlyBase, 2016)

    3-5.8

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (29)
    Genomic Clones (17)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (1)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
              Other Comments

              Isolated in a screen for seminal fluid proteins.

              (Mueller et al., 2004)

              Gene is identified in a differential cDNA hybridisation screen.

              (Wolfner et al., 1997)
              Relationship to Other Genes
              Source for database merge of
              Additional comments
              Nomenclature History
              Source for database identify of
              Nomenclature comments
              Etymology
              Synonyms and Secondary IDs (7)
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              Study result (0)
              Result
              Result Type
              Title
              External Crossreferences and Linkouts ( 86 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              Other crossreferences
              AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
              DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
              EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
              FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
              FlyMine - An integrated database for Drosophila genomics
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              MARRVEL_MODEL - MARRVEL (model organism gene)
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              Flygut - An atlas of the Drosophila adult midgut
              MIST (protein-protein) - An integrated Molecular Interaction Database
              References (92)