FB2020_06, released Dec 22, 2020

Gene: Dmel\Acp62F

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General Information
Symbol
Dmel\Acp62F
Species
D. melanogaster
Name
Accessory gland protein 62F
Annotation Symbol
CG1262
Feature Type
protein_coding_gene
FlyBase ID
FBgn0020509
Gene Model Status
Current
Stock Availability
6 publicly available
Gene Snapshot
Accessory gland protein 62F (Acp62F) encodes a trypsin inhibitor that is synthesized in male accessory glands and transferred to females during mating. It influences sperm storage in mated females. [Date last reviewed: 2019-03-07]
Other Summaries
Also Known As

Accessory gland peptide 62F

Key Links
Genomic Location
Cytogenetic map
62F2-62F2
Sequence location
3L:2,739,750..2,740,228 [+]
Recombination map

3-4

RefSeq locus
NT_037436 REGION:2739750..2740228
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables serine-type endopeptidase inhibitor activity
inferred from direct assay
(Lung et al., 2002)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in multicellular organism reproduction
inferred from high throughput expression pattern
(Findlay et al., 2009, Findlay et al., 2008, Mueller et al., 2005)
involved_in negative regulation of proteolysis
inferred from direct assay
(Lung et al., 2002)
inferred from mutant phenotype
(Mueller et al., 2008)
involved_in sperm competition
inferred from mutant phenotype
(Mueller et al., 2008)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in extracellular space
inferred from direct assay
(Lung et al., 2002)
inferred from high throughput direct assay
(Findlay et al., 2009, Findlay et al., 2008)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Protein Function (UniProtKB)
Responsible for physiological and behavioral changes in mated female flies. May contribute to the toxicity of seminal fluid and the decreased life-span of mated females. May also affect neuromuscular events after mating concerning sperm storage and egg release.
(UniProt, O46202)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Acp62F for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Acp62F-RA
FBtr0072943
NM_079168
479
115
Additional Transcript Data and Comments
Reported size (kB)

0.45 (northern blot)

(Wolfner et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Acp62F-PA
FBpp0072820
12.6
115
7.36
NP_523892
AAF47683
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

115 (aa)

(Wolfner et al., 1997)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Acp62F using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
female reproductive system

Comment: mated females

(Ravi Ram et al., 2005)
adult hemolymph

Comment: mated females

(Ravi Ram et al., 2005)
uterus

Comment: mated females

(Ravi Ram et al., 2005)
spermathecum

Comment: mated females

(Ravi Ram et al., 2005)
seminal receptacle

Comment: mated females

(Ravi Ram et al., 2005)
oviduct

Comment: mated females

(Ravi Ram et al., 2005)
ovary

Comment: mated females

(Ravi Ram et al., 2005)
oocyte

Comment: mated females

(Ravi Ram et al., 2005)
egg

Comment: mated females

(Ravi Ram et al., 2005)
Additional Descriptive Data

Acp62F is transferred to the female at the time of mating and persists for about 2-3 hours in the female reproductive tract. Acp62F enters the female hemolymph. It is detected in the uterus, sperm mass, spermathecum, seminal receptacle, oviduct, ovary, mature oocyte, and eggs.

(Ravi Ram et al., 2005)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in extracellular space
inferred from direct assay
(Lung et al., 2002)
inferred from high throughput direct assay
(Findlay et al., 2009, Findlay et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Acp62F in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Acp62F
Transgenic constructs containing regulatory region of Acp62F
Deletions and Duplications ( 1 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4αTub84B.PL
lethal | heat sensitive, with Scer\GAL4hs.PB
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (0)
No records found.
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (0)
No records found.
Rattus norvegicus (Norway rat) (0)
No records found.
Xenopus tropicalis (Western clawed frog) (11)
Xtro\LOC100487636
1 of 12
Yes
No
Xtro\LOC100487808
1 of 12
Yes
Yes
Xtro\LOC100487969
1 of 12
Yes
Yes
Xtro\LOC100493186
1 of 12
Yes
Yes
Xtro\LOC100494280
1 of 12
Yes
Yes
Xtro\LOC101732767
1 of 12
Yes
Yes
Xtro\LOC101733006
1 of 12
Yes
Yes
Xtro\LOC101733954
1 of 12
Yes
Yes
Xtro\LOC101734536
1 of 12
Yes
Yes
Xtro\LOC105947976
1 of 12
Yes
No
Xtro\LOC108648061
1 of 12
Yes
No
Danio rerio (Zebrafish) (0)
No records found.
Caenorhabditis elegans (Nematode, roundworm) (15)
Cele\F53C11.9
4 of 15
Yes
No
Cele\C10G8.4
3 of 15
No
No
Cele\swm-1
3 of 15
No
No
Cele\B0238.12
2 of 15
No
No
Cele\C25E10.7
2 of 15
No
No
Cele\C25E10.8
2 of 15
No
No
Cele\C25E10.10
2 of 15
No
No
Cele\F36H9.4
2 of 15
No
No
Cele\Y26D4A.12
2 of 15
No
No
Cele\C53B7.2
1 of 15
No
No
Cele\F47G3.4
1 of 15
No
Yes
Cele\spi-1
1 of 15
No
Yes
Cele\T06E6.10
1 of 15
No
Yes
Cele\Y69H2.3
1 of 15
No
Yes
Cele\Y69H2.10
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190ITB )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila simulans
Dsim\Acp62F
Drosophila sechellia
Dsec\Acp62F
Drosophila erecta
Dere\GG14885
Drosophila yakuba
Dyak\GE20336
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150FJ1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ014781
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0OUQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA009092
Bombyx mori
Silkmoth
Bmor\BGIBMGA009095
Bombyx mori
Silkmoth
Bmor\Bmo.12667
Bombyx mori
Silkmoth
Bmor\BGIBMGA009075
Bombyx mori
Silkmoth
Bmor\BGIBMGA009091
Bombyx mori
Silkmoth
Bmor\BGIBMGA010890
Bombyx mori
Silkmoth
Bmor\BGIBMGA009073
Bombyx mori
Silkmoth
Bmor\BGIBMGA010891
Bombyx mori
Silkmoth
Bmor\BGIBMGA010889
Bombyx mori
Silkmoth
Bmor\BGIBMGA010892
Bombyx mori
Silkmoth
Bmor\BGIBMGA004727
Bombyx mori
Silkmoth
Bmor\BGIBMGA009093
Bombyx mori
Silkmoth
Bmor\BGIBMGA009094
Bombyx mori
Silkmoth
Bmor\BGIBMGA009072
Bombyx mori
Silkmoth
Bmor\BGIBMGA004728
Danaus plexippus
Monarch butterfly
Dple\DPOGS207577-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS201004-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS201007-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS210758-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL004886
Heliconius melpomene
Postman butterfly
Hmel\HMEL011567
Heliconius melpomene
Postman butterfly
Hmel\HMEL018084
Heliconius melpomene
Postman butterfly
Hmel\HMEL013716
Heliconius melpomene
Postman butterfly
Hmel\HMEL004885
Heliconius melpomene
Postman butterfly
Hmel\HMEL013718
Heliconius melpomene
Postman butterfly
Hmel\HMEL006528
Apis florea
Little honeybee
Aflo\5026882020
Apis florea
Little honeybee
Aflo\2278767492
Apis florea
Little honeybee
Aflo\2167977586
Apis florea
Little honeybee
Aflo\5026881956
Apis florea
Little honeybee
Aflo\5026881988
Apis florea
Little honeybee
Aflo\2147484625
Apis florea
Little honeybee
Aflo\2629969988
Apis florea
Little honeybee
Aflo\2171085538
Apis mellifera
Western honey bee
Amel\LOC100576444
Apis mellifera
Western honey bee
Amel\GB50121
Apis mellifera
Western honey bee
Amel\GB50120
Apis mellifera
Western honey bee
Amel\GB50116
Apis mellifera
Western honey bee
Amel\LOC725163
Apis mellifera
Western honey bee
Amel\GB41374
Apis mellifera
Western honey bee
Amel\GB49509
Apis mellifera
Western honey bee
Amel\Ame.9999
Apis mellifera
Western honey bee
Amel\LOC725114
Apis mellifera
Western honey bee
Amel\GB49510
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP15742
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP15740
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP15741
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP15645
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP24912
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP16573
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP15646
Bombus terrestris
Buff-tailed bumblebee
Bter\15210819
Bombus terrestris
Buff-tailed bumblebee
Bter\15206073
Bombus terrestris
Buff-tailed bumblebee
Bter\15210818
Bombus terrestris
Buff-tailed bumblebee
Bter\15210816
Bombus terrestris
Buff-tailed bumblebee
Bter\15208173
Bombus terrestris
Buff-tailed bumblebee
Bter\15210209
Bombus terrestris
Buff-tailed bumblebee
Bter\15210817
Bombus terrestris
Buff-tailed bumblebee
Bter\15210813
Bombus terrestris
Buff-tailed bumblebee
Bter\15210815
Bombus terrestris
Buff-tailed bumblebee
Bter\15202758
Linepithema humile
Argentine ant
Lhum\LH21323
Linepithema humile
Argentine ant
Lhum\LH18394
Linepithema humile
Argentine ant
Lhum\LH21694
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002c98
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002c94
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002c9a
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000760
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00291b
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002c99
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000352
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000378
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000379
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000280
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000380
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000354
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000351
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG004029
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG010107
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000359
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_02967
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00932
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_02968
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00933
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_04773
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08733
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_01026
Tribolium castaneum
Red flour beetle
Tcas\TC007946
Tribolium castaneum
Red flour beetle
Tcas\TC007945
Tribolium castaneum
Red flour beetle
Tcas\TC032774
Tribolium castaneum
Red flour beetle
Tcas\TC033045
Tribolium castaneum
Red flour beetle
Tcas\TC032406
Tribolium castaneum
Red flour beetle
Tcas\TC032363
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0JD8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Tetranychus urticae
Two-spotted spider mite
Turt\tetur22g00260
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (8)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG33259
4 of 10
CG5267
2 of 10
CG34189
2 of 10
CG42259
2 of 10
CG42878
1 of 10
CG43229
1 of 10
CG45546
1 of 10
Sfp53D
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map

    3-4

    Cytogenetic map
    62F2-62F2
    Sequence location
    3L:2,739,750..2,740,228 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    62F2-62F2
    Limits computationally determined from genome sequence between P{PZ}Spn06911 and P{PZ}l(3)0680306803
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    62F-62F
    (determined by in situ hybridisation)
    (Wolfner et al., 1997)
    Experimentally Determined Recombination Data
    Location

    3-4

    (FlyBase, 2016)

    3-5.8

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (6)
    Genomic Clones (17)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (1)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of
          Source for database merge of
          Additional comments
          Other Comments

          Isolated in a screen for seminal fluid proteins.

          (Mueller et al., 2004)

          Gene is identified in a differential cDNA hybridisation screen.

          (Wolfner et al., 1997)
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 85 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Synonyms and Secondary IDs (7)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (87)