FlyBase Gene Report: Dmel\Sgp

General Information

Symbol

Dmel\Sgp

Species

D. melanogaster

Name

Sgroppino

Annotation Symbol

CG13091

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032055

Gene Model Status

Current

Stock Availability

9 publicly available

Enzyme Name (EC)

alcohol-forming fatty acyl-CoA reductase (NADPH) (1.2.1.84)

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

29D4-29D4

Sequence location

2L:8,519,690..8,521,809 [+]

Recombination map

2-33

RefSeq locus

NT_033779 REGION:8519690..8521809

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables alcohol-forming long-chain fatty acyl-CoA reductase activity

inferred from sequence or structural similarity with UniProtKB:Q8WVX9

(Gene Ontology Curators, 2002-)

enables alcohol-forming very long-chain fatty acyl-CoA reductase activity

inferred from electronic annotation with InterPro:IPR026055

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000110226

(GO Reference Genome Project, 2011-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in long-chain fatty-acyl-CoA metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000110226

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in peroxisomal membrane

inferred from mutant phenotype

(Merkling et al., 2019)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in peroxisomal membrane

inferred from biological aspect of ancestor with PANTHER:PTN000110227

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

FATTY ACYL-COA REDUCTASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

alcohol-forming long-chain fatty acyl-CoA reductase activity

a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA (1.2.1.84)

RHEA 52716: alcohol-forming very long-chain fatty acyl-CoA reductase activity
RHEA 81751:

Summaries

Gene Group (FlyBase)

FATTY ACYL-COA REDUCTASES -: Fatty acyl-CoA reductases (FARs) catalyze the reduction of acyl-CoA to alcohols and aldehydes. In insects, they are involved in synthesis of cuticular hydrocarbons. (Adapted from FBrf0242506.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Sgp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VLJ7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Sgp-RA

FBtr0079684

NM_135397

1718

523

Sgp-RB

FBtr0333415

NM_001273328

1796

523

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Sgp-PA

FBpp0079296

59.5

523

9.36

FBpp0305607

59.5

523

9.36

Q9VLJ7

NP_001260257

AGB92793

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

523 aa isoforms: Sgp-PA, Sgp-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VLJ7

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sgp using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG13091_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.13

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult fat body

Comment: cyclical, peak ZT21-0

(Xu et al., 2011)

Additional Descriptive Data

Expressed cyclically in the adult fat body.

(Xu et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in peroxisomal membrane

inferred from mutant phenotype

(Merkling et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Sgp in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Sgp

View exonic expression by tissue for Dmel\Sgp

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032055

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34188/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032055

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032055

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032055

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13091

Flygut - An atlas of the Drosophila adult midgut

FBgn0032055

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Sgp

Transgenic constructs containing regulatory region of Sgp

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel7038

(Ryder, 2004.4.26)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Sgp^GD1010

viable, with Scer\GAL4^Mef2.PR

Sgp^GD1010

viable, with Scer\GAL4^pnr-MD237

Sgp^GD1010

viable, with Scer\GAL4^tin.CΔ4

Sgp^GD1010

Other Phenotypes

Allele

abnormal immune response | adult stage

Sgp^EY06744

abnormal immune response | adult stage, with Scer\GAL4^Act.PU

Sgp^KK106304

increased mortality | adult stage, with Scer\GAL4^Act.PU

Sgp^KK106304

increased mortality | adult stage | conditional

Sgp^EY06744

visible | adult stage

Sgp^EY06744

Phenotype manifest in

Allele

adult abdominal fat mass

Sgp^EY06744

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (6)

Hsap\FAR2

7 of 14

Yes

6 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (7)

Rnor\Far2

7 of 14

Yes

6 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (11)

Mmus\Far2

7 of 14

Yes

6 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (5)

Xtro\far1

5 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (2)

Drer\si:dkey-97m3.1

7 of 14

Yes

Drer\far1

5 of 14

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\fard-1

6 of 14

Yes

Anopheles gambiae (African malaria mosquito) (16)

Agam\AgaP_AGAP001343

7 of 12

Yes

Agam\AgaP_AGAP005985

7 of 12

Yes

Agam\AgaP_AGAP005986

7 of 12

Yes

Agam\AgaP_AGAP011736

7 of 12

Yes

Agam\AgaP_AGAP005984

6 of 12

Yes

Agam\AgaP_AGAP010785

6 of 12

Yes

6 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

3 of 12

Arabidopsis thaliana (thale-cress) (15)

Atha\CER4

10 of 13

Yes

Atha\FAR4

10 of 13

Yes

Atha\FAR5

10 of 13

Yes

Atha\FAR6

10 of 13

Yes

Atha\FAR7

10 of 13

Yes

Atha\FAR8

10 of 13

Yes

Atha\MS2

10 of 13

Yes

Atha\FAR1

9 of 13

Atha\AT3G60060

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\LYS2

2 of 13

Yes

Scer\ERG26

1 of 13

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\lys1

2 of 12

Yes

Spom\SPCC162.02c

2 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Sgp. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (17)

11 of 13

11 of 13

11 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

wat

10 of 13

9 of 13

9 of 13

8 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

FAR2; fatty acyl-CoA reductase 2

7 of 14

616156

FAR1; fatty acyl-CoA reductase 1

6 of 14

616107

rhizomelic chondrodysplasia punctate type 4

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0032055

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

plasmalogen biosynthesis I (aerobic)

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Wax biosynthesis

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Peroxisome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Wax biosynthesis

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-33

Cytogenetic map

29D4-29D4

Sequence location

2L:8,519,690..8,521,809 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

29D4-29D4

Limits computationally determined from genome sequence between P{lacW}l(2)k03706^k03706&P{lacW}Acer^k07704 and P{PZ}lmg⁰³⁴²⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-33

(FlyBase, 2016)

2-28.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

96289

y¹ w^*; P{TKO-UAS.GS05941}attP40

15973

y¹ w^67c23; P{EPgy2}Sgp^EY06744

605414

y¹ w^*; TI{CRIMIC.TG4.0}Sgp^{CR70893-TG4.0}

NIG-Fly

HMJ23496

y¹ v¹; P{TRiP.HMJ23496}attP40/CyO

M2L-0286

y¹ w¹¹¹⁸; Sgp^SK1 P{neoFRT}40A/CyO-Tb

M2L-0287

y¹ w¹¹¹⁸; Sgp^SK4 P{neoFRT}40A/CyO-Tb

2LG-0233

y² VhaAC39-1^cho-1 v¹; P{U6-Sgp.NIG.sgRNA}attP40/CyO

VDRC

v100943

P{KK106304}VIE-260B

v11729

w¹¹¹⁸; P{GD1010}v11729

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (2)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH27892

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032055

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

Dmel_neb_009613_0694_2091

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032055

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Sgp CG13091

(Merkling et al., 2019)

Nomenclature comments

Etymology

Named 'Sgroppino' because, after removal of the digestive track and reproductive system, a relatively larger mass of fat body tissue remains loosely attached to the abdominal carcass in mutants, forming large oleaginous droplets and appearing white - reminiscent of the Sgroppino cocktail.

(Merkling et al., 2019)

Synonyms and Secondary IDs (5)

Reported As

Name Synonyms

Sgroppino

(Merkling et al., 2019)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 38 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34188

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VLJ7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VLJ7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VLJ7

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032055

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34188/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032055

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032055

FlyMine - An integrated database for Drosophila genomics

FBgn0032055

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG13091

MARRVEL_MODEL - MARRVEL (model organism gene)

34188

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032055

DroID - A comprehensive database of gene and protein interactions.

FBgn0032055

DRSC - Results frm RNAi screens

FBgn0032055

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG13091_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032055

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032055

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

plasmalogen biosynthesis I (aerobic)

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13091

Flygut - An atlas of the Drosophila adult midgut

FBgn0032055

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Peroxisome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Wax biosynthesis

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Wax biosynthesis

References (44)

The following external sites may use different assemblies or annotations than FlyBase.

-0.13

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