FlyBase Gene Report: Dmel\Ing5

General Information

Symbol

Dmel\Ing5

Species

D. melanogaster

Name

Inhibitor of growth family member 5

Annotation Symbol

CG9293

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032516

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

Inhibitor of growth family member 5 (Ing5) encodes a histone acetyltransferase that forms part of the Enok complex, which interacts with the product of elg1 to promote the G1/S transition via proliferating cell nuclear antigen (PCNA) unloading. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

34B9-34B9

Sequence location

2L:13,385,848..13,387,312 [-]

Recombination map

2-48

RefSeq locus

NT_033779 REGION:13385848..13387312

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables acetyltransferase activator activity

inferred from direct assay

(Huang et al., 2016)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of DNA-templated transcription

inferred from biological aspect of ancestor with PANTHER:PTN000035903

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of MOZ/MORF histone acetyltransferase complex

inferred from direct assay

(Huang et al., 2016)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000817813

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ENOK COMPLEX

Protein Family (UniProt)

Belongs to the ING family. (Q9VJY8)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

ENOK COMPLEX -: The Enok complex (Enok-C) is an histone lysine acetyltransferase 6 (KAT6) complex. The Enok-C preferentially acetylates histone H3 residues. KAT6 complexes are highly conserved in eukaryotes and are involved in cell cycle regulation. (Adapted from FBrf0232456).

Protein Function (UniProtKB)

Component of the Enok complex which has a histone H3 acetyltransferase activity.

(UniProt, Q9VJY8)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ing5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VJY8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.51

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ing5-RA

FBtr0080525

NM_135803

1044

285

Ing5-RB

FBtr0080526

NM_165042

1002

271

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ing5-PA

FBpp0080103

32.1

285

8.71

FBpp0080104

30.5

271

8.38

Q8IP71

NP_723814

AAN10835

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the Enok complex composed of at least Br140, enok, Eaf6 and Ing5.

(UniProt, Q9VJY8)

Domain

The PHD-type zinc finger mediates the binding to H3K4me3.

(UniProt, Q9VJY8)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ing5 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG9293_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.33

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

central brain primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of MOZ/MORF histone acetyltransferase complex

inferred from direct assay

(Huang et al., 2016)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ing5 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ing5

View exonic expression by tissue for Dmel\Ing5

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032516

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34752/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032516

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032516

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032516

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9293

Flygut - An atlas of the Drosophila adult midgut

FBgn0032516

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ing5

Transgenic constructs containing regulatory region of Ing5

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)loqs^del

(Ghildiyal et al., 2006)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Ing5^EY13364

Sterility

Allele

fertile

Ing5^EY13364

male fertile, with Scer\GAL4^bam.PU

Ing5^KK108992

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (11)

Hsap\ING4

13 of 14

Yes

Hsap\ING5

13 of 14

Yes

5 of 14

5 of 14

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\TAF3

1 of 14

Hsap\ZMYND8

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (10)

Rnor\Ing4

13 of 14

Yes

Rnor\Ing5

12 of 14

Yes

5 of 14

5 of 14

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (9)

Mmus\Ing4

13 of 14

Yes

Mmus\Ing5

12 of 14

Yes

5 of 14

5 of 14

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (6)

Xtro\ing4

11 of 13

Yes

Xtro\ing5

9 of 13

Yes

4 of 13

4 of 13

3 of 13

1 of 13

Danio rerio (Zebrafish) (12)

Drer\ing4

13 of 14

Yes

Drer\ing5a

13 of 14

Yes

Drer\ing5b

13 of 14

Yes

5 of 14

5 of 14

4 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC101884644

1 of 14

Drer\phf10

1 of 14

Drer\taf3

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\lsy-13

8 of 14

Yes

Cele\ing-3

7 of 14

Cele\Y43H11AL.1

3 of 14

Yes

Cele\athp-3

1 of 14

Yes

Cele\phf-31

1 of 14

Yes

Cele\phf-34

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (5)

Agam\AgaP_AGAP009992

12 of 12

Yes

5 of 12

5 of 12

2 of 12

2 of 12

Arabidopsis thaliana (thale-cress) (5)

Atha\ING1

11 of 13

Yes

Atha\ING2

7 of 13

Atha\AT5G12400

1 of 13

Yes

Atha\AT5G22760

1 of 13

Yes

Atha\AT5G58610

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\PHO23

8 of 13

Yes

Scer\YNG1

6 of 13

Yes

Scer\YNG2

6 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\png2

8 of 12

Yes

Spom\png1

6 of 12

Spom\spf1

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ing5. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (5)

Ing3

10 of 13

CG7379

9 of 13

MESR4

2 of 13

pps

1 of 13

Taf3

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ING4; inhibitor of growth family member 4

13 of 14

608524

ING5; inhibitor of growth family member 5

13 of 14

608525

ING1; inhibitor of growth family member 1

5 of 14

601566

SQUAMOUS CELL CARCINOMA, HEAD AND NECK; HNSCC

head and neck squamous cell carcinoma

ING2; inhibitor of growth family member 2

5 of 14

604215

ING3; inhibitor of growth family member 3

5 of 14

607493

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ing5 - Br140

anti tag coimmunoprecipitation, peptide massfingerprinting, cross-linking study, tandem affinity purification, Identification by mass spectrometry

(Kang et al., 2017, Huang et al., 2016)

Ing5 - Eaf6

anti tag coimmunoprecipitation, peptide massfingerprinting

(Huang et al., 2016)

Ing5 - Tctp

pull down, anti tag western blot, anti tag coimmunoprecipitation

(Kim et al., 2023)

Ing5 - elg1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Huang et al., 2016)

Ing5 - enok

anti tag coimmunoprecipitation, peptide massfingerprinting

(Huang et al., 2016)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Component of the Enok complex composed of at least Br140, enok, Eaf6 and Ing5.

(UniProt, Q9VJY8 )

Linkouts

BioGRID - A database of protein and genetic interactions.

60787

DroID - A comprehensive database of gene and protein interactions.

FBgn0032516

MIST (protein-protein) - An integrated Molecular Interaction Database

34752

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Regulation of TP53 Activity through Acetylation

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-48

Cytogenetic map

34B9-34B9

Sequence location

2L:13,385,848..13,387,312 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34B9-34B9

Limits computationally determined from genome sequence between P{lacW}l(2)k00302^k00302 and P{PZ}kuz⁰³⁷⁸²&P{lacW}kuz^k14817

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-48

(FlyBase, 2016)

2-43.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

18705

w¹¹¹⁸; PBac{WH}Ing5^f03884/CyO

20873

y¹ w^67c23; P{EPgy2}Ing5^EY13364

80637

y¹ w^*; TI{CRIMIC.TG4.0}Ing5^{CR00744-TG4.0}/SM6a

91570

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; Ing5¹⁴⁵ P{neoFRT}40A/CyO; P{PRE.Scr-PS1}J3 P{PRE.Scr-PS1}Q1

FlyORF

F002850

M{UAS-Ing5.ORF.3xHA.GW}ZH-86Fb

Kyoto

114654

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; PBac{WH}Ing5^f03884 P{neoFRT}40A/CyO y⁺

NIG-Fly

M2L-2139

y¹ w¹¹¹⁸; Ing5^SK1 P{neoFRT}40A/CyO-Tb

M2L-2140

y¹ w¹¹¹⁸; Ing5^SK7 P{neoFRT}40A/CyO-Tb

2LG-1699

y² VhaAC39-1^cho-1 v¹; P{U6-Ing5.NIG.sgRNA}attP40/CyO

VDRC

v102002

P{KK108992}VIE-260B

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (33)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032516

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032516

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Kim et al., 2023)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Ing5 CG9293

(Huang et al., 2016)

Nomenclature comments

Etymology

Named 'Ing5' based on the nomenclature of the human ortholog.

(Huang et al., 2016)

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG9293

(National Institute of Genetics Fly Stocks, 2022-, Huang et al., 2016, Izutsu et al., 2015, Kim and Orr-Weaver, 2011, Keleman et al., 2009.8.5, Estes et al., 2008, Bienz, 2006, Zarnescu et al., 2005)

Ing5

(François et al., 2023, Kim et al., 2023, Kennison, 2020.10.22, Levis, 2018.8.30, Huang et al., 2016)

Name Synonyms

Inhibitor of growth family member 5

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34752

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VJY8

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VJY8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VJY8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032516

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34752/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032516

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032516

FlyMine - An integrated database for Drosophila genomics

FBgn0032516

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9293

MARRVEL_MODEL - MARRVEL (model organism gene)

34752

Linkouts

BioGRID - A database of protein and genetic interactions.

60787

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032516

DroID - A comprehensive database of gene and protein interactions.

FBgn0032516

DRSC - Results frm RNAi screens

FBgn0032516

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG9293_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032516

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032516

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9293

Flygut - An atlas of the Drosophila adult midgut

FBgn0032516

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032516

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013703

MIST (protein-protein) - An integrated Molecular Interaction Database

34752

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Regulation of TP53 Activity through Acetylation

References (36)

The following external sites may use different assemblies or annotations than FlyBase.

1.33

Report Sections