FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\CG32354
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General Information
Symbol
Dmel\CG32354
Species
D. melanogaster
Name
Annotation Symbol
CG32354
Feature Type
protein_coding_gene
FlyBase ID
FBgn0052354
Gene Model Status
Current
Stock Availability
4 publicly available
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
66C11-66C12
Sequence location
Recombination map
3-24
RefSeq locus
NT_037436 REGION:8415857..8422205
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (2 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in cell differentiation
inferred from biological aspect of ancestor with PANTHER:PTN000099219
(GO Reference Genome Project, 2011-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in extracellular region
inferred from biological aspect of ancestor with PANTHER:PTN000099217
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\CG32354 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VSK1)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
CG32354-RA
FBtr0076662
NM_168275
3251
662
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
CG32354-PA
FBpp0076385
71.9
662
8.28
Q9VSK1
NP_729380
AAF50418
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG32354 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.38

Transcript Expression
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG32354 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 9-10
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of CG32354
Transgenic constructs containing regulatory region of CG32354
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 4 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
lethal - all die during embryonic stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (22)
Hsap\SPINK5
5 of 14
Yes
Yes
Hsap\SPINK1
3 of 14
No
No
Hsap\FST
2 of 14
No
No
3  
Hsap\FSTL3
2 of 14
No
No
Hsap\SPINK2
2 of 14
No
No
Hsap\SPINK4
2 of 14
No
No
Hsap\SPINK6
2 of 14
No
Yes
Hsap\SPINK7
2 of 14
No
No
1  
Hsap\SPINK9
2 of 14
No
No
Hsap\SPINK14
2 of 14
No
No
Hsap\TMEFF1
2 of 14
No
Yes
Hsap\TMEFF2
2 of 14
No
Yes
Hsap\AGRN
1 of 14
No
No
Hsap\FSTL5
1 of 14
No
Yes
Hsap\HTRA1
1 of 14
No
No
Hsap\HTRA3
1 of 14
No
No
Hsap\HTRA4
1 of 14
No
No
Hsap\KAZALD1
1 of 14
No
Yes
Hsap\MSANTD3-TMEFF1
1 of 14
No
Yes
Hsap\RECK
1 of 14
No
No
Hsap\SPINK8
1 of 14
No
No
Hsap\SPINK13
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (22)
Rnor\Spink5
5 of 14
Yes
Yes
Rnor\Fst
2 of 14
No
No
Rnor\Spink1l
2 of 14
No
No
Rnor\Spink1
2 of 14
No
No
Rnor\Spink2
2 of 14
No
No
Rnor\Spink6
2 of 14
No
No
Rnor\Spink10
2 of 14
No
Yes
Rnor\Spink13
2 of 14
No
Yes
Rnor\Spink14
2 of 14
No
No
Rnor\Tmeff1
2 of 14
No
Yes
Rnor\Agrn
1 of 14
No
No
Rnor\Fstl3
1 of 14
No
No
Rnor\Fstl4
1 of 14
No
Yes
Rnor\Fstl5
1 of 14
No
Yes
Rnor\Htra1
1 of 14
No
No
Rnor\Reck
1 of 14
No
No
Rnor\RGD1559536
1 of 14
No
No
Rnor\Spink4
1 of 14
No
No
Rnor\Spink7
1 of 14
No
No
Rnor\Spink8
1 of 14
No
No
Rnor\Spink9
1 of 14
No
No
Rnor\Tmeff2
1 of 14
No
Yes
Mus musculus (laboratory mouse) (25)
Mmus\Spink5
4 of 14
Yes
Yes
Mmus\Fst
2 of 14
No
No
Mmus\Spink1
2 of 14
No
No
Mmus\Spink2
2 of 14
No
No
Mmus\Spink4
2 of 14
No
No
Mmus\Spink6
2 of 14
No
No
Mmus\Spink7
2 of 14
No
No
Mmus\Spink12
2 of 14
No
No
Mmus\Spink13
2 of 14
No
Yes
Mmus\Spink14
2 of 14
No
No
Mmus\Tmeff1
2 of 14
No
No
Mmus\Tmeff2
2 of 14
No
Yes
Mmus\Agrn
1 of 14
No
No
Mmus\Fstl3
1 of 14
No
No
Mmus\Fstl4
1 of 14
No
Yes
Mmus\Fstl5
1 of 14
No
Yes
Mmus\Gm10267
1 of 14
No
No
Mmus\Gm36368
1 of 14
No
No
Mmus\Htra1
1 of 14
No
No
Mmus\Reck
1 of 14
No
No
Mmus\Slco6d1
1 of 14
No
No
Mmus\Spink8
1 of 14
No
Yes
Mmus\Spink10
1 of 14
No
No
Mmus\Spink11
1 of 14
No
No
Mmus\Spinkl
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (11)
Xtro\LOC100494615
2 of 13
Yes
Yes
Xtro\agrn
1 of 13
No
No
Xtro\fat1
1 of 13
No
No
Xtro\fstl3
1 of 13
No
No
Xtro\fst
1 of 13
No
No
Xtro\htra1
1 of 13
No
No
Xtro\htra4
1 of 13
No
No
Xtro\jkamp
1 of 13
No
No
Xtro\slco5a1
1 of 13
No
No
Xtro\smoc1
1 of 13
No
No
Xtro\tmeff1
1 of 13
No
Yes
Danio rerio (Zebrafish) (24)
Drer\agrn
3 of 14
Yes
Yes
Drer\fsta
2 of 14
No
No
Drer\fstb
2 of 14
No
No
Drer\si:ch211-195b11.3
2 of 14
No
No
Drer\spink2.5
2 of 14
No
No
Drer\spink4
2 of 14
No
No
Drer\tmeff1a
2 of 14
No
Yes
Drer\tmeff1b
2 of 14
No
Yes
Drer\fstl5
1 of 14
No
Yes
Drer\htra1b
1 of 14
No
No
Drer\htra4
1 of 14
No
No
Drer\slco1c1
1 of 14
No
No
Drer\smoc1
1 of 14
No
No
Drer\spink2.1
1 of 14
No
No
Drer\spink2.2
1 of 14
No
No
Drer\spink2.3
1 of 14
No
No
Drer\spink2.4
1 of 14
No
No
Drer\spink2.6
1 of 14
No
No
Drer\spink2.7
1 of 14
No
No
Drer\spink2.8
1 of 14
No
No
Drer\spink2.9
1 of 14
No
No
Drer\spink2.10
1 of 14
No
No
Drer\spink2.11
1 of 14
No
No
Drer\tmeff2a
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\agr-1
4 of 14
Yes
Yes
Cele\F29G6.1
4 of 14
Yes
Yes
Cele\ZK813.6
3 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (12)
Agam\AgaP_AGAP005450
12 of 12
Yes
Yes
Agam\AgaP_AGAP006282
2 of 12
No
No
Agam\AgaP_AGAP006739
1 of 12
No
No
Agam\AgaP_AGAP007906
1 of 12
No
No
Agam\AgaP_AGAP007907
1 of 12
No
No
Agam\AgaP_AGAP008968
1 of 12
No
No
Agam\AgaP_AGAP009327
1 of 12
No
No
Agam\AgaP_AGAP009328
1 of 12
No
No
Agam\AgaP_AGAP009527
1 of 12
No
No
Agam\AgaP_AGAP010646
1 of 12
No
No
Agam\AgaP_AGAP011482
1 of 12
No
No
Agam\AgaP_AGAP012576
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:CG32354. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (14)
Fs
3 of 13
CG12716
2 of 13
Reck
2 of 13
CG1077
1 of 13
CG31704
1 of 13
CG42486
1 of 13
CG44008
1 of 13
CG44090
1 of 13
CG45011
1 of 13
CG45012
1 of 13
Kaz-m1
1 of 13
magu
1 of 13
Oatp58Dc
1 of 13
Sfp33A3
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    SPINK5; serine peptidase inhibitor Kazal type 5
    model of  Netherton syndrome
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    SPINK5; serine peptidase inhibitor Kazal type 5
    5 of 14
    605010
    SPINK1; serine peptidase inhibitor Kazal type 1
    3 of 14
    167790
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Interaction Browsers
    Summary of Genetic Interactions
    Interaction Browsers
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map
    3-24
    Cytogenetic map
    66C11-66C12
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    66C11-66C12
    Limits computationally determined from genome sequence between P{lacW}l(3)L0139L0139 and P{PZ}Gug03928&P{EP}CG6745EP595b
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    3-24

    (FlyBase, 2016)

    3-19.7

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (4)
    Genomic Clones (11)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (24)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            Identification: cDNA screen for secreted and transmembrane proteins expressed during embryogenesis. Identified with: CK01670 <up>FlyBase curator comment: EST subsequently found to be chimeric</up>.

            (Kopczynski et al., 1998)

            EST CK01670 is chimeric; the 5' portion corresponds to part of CG3777, the 3' portion corresponds to part of CG32354.

            (FlyBase, 1996-)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: CG7027 CG7159

            (FlyBase, 1996-)
            Additional comments

            Annotations CG7027, CG7159 and CG17355 merged as CG32354 in release 3 of the genome annotation.

            (FlyBase Genome Annotators, 2002-2003)

            Source for merge of CG7027 CG7159 was a shared cDNA ( date:010720 ).

            (FlyBase, 1996-)
            Nomenclature History
            Source for database identify of
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (6)
            Reported As
            Symbol Synonym
            BEST:CK01670
            CG17355
            (Ren et al., 2005, Luschnig, 2003.12.17)
            CG32354
            (Ni et al., 2010.12.1, Lebo et al., 2009, Firth and Baker, 2007)
            CG7027
            CG7159
            CK01670
            (Kopczynski et al., 1998)
            Name Synonyms
            Secondary FlyBase IDs
            • FBgn0025695
            • FBgn0035893
            • FBgn0040829
            • FBgn0040830
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 33 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            References (33)