FlyBase Gene Report: Dmel\CG33217

General Information

Symbol

Dmel\CG33217

Species

D. melanogaster

Name

Annotation Symbol

CG33217

Feature Type

protein_coding_gene

FlyBase ID

FBgn0053217

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

80D2-80D2

Sequence location

3L:23,126,025..23,132,209 [+]

Recombination map

3-47

RefSeq locus

NT_037436 REGION:23126025..23132209

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse JBrowse2 (beta)

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in rRNA processing

inferred from biological aspect of ancestor with PANTHER:PTN002147636

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002147636

(GO Reference Genome Project, 2011-)

Gene Group / Complex (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Armadillo-type fold

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG33217 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q75G43)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

RNA-seq exon Junction FBsf0000179261 not annotated. It connects to a duplicated partial downstream copy of the CG33217. The duplication may be a genome assembly error.

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG33217-RA

FBtr0070045

NM_206425

2227

699

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG33217-PA

FBpp0070044

80.0

699

8.19

Q8MS38

NP_996147

AAS64918

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Armadillo-type fold

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG33217 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG33217_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.42

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

germline cell

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

germline cell

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

circular intestinal visceral muscle fiber

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval visceral muscle cell

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG33217 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG33217

View exonic expression by tissue for Dmel\CG33217

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0053217

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

2768951/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0053217

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0053217

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0053217

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33217

Flygut - An atlas of the Drosophila adult midgut

FBgn0053217

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CG33217

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG33217

Transgenic constructs containing regulatory region of CG33217

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG33217^GD16022

lethal, with Scer\GAL4^Tub.PU

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG33217^GD16022

partially lethal, with Scer\GAL4^tin.CΔ4

CG33217^GD16022

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\PELP1

5 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Pelp1

6 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Pelp1

4 of 14

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\pelp1

5 of 13

Yes

Danio rerio (Zebrafish) (1)

Drer\pelp1

7 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP003842

11 of 12

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\AT1G30240

4 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\RIX1

2 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPCC4G3.18

2 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG33217. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

CG11629

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PELP1; proline, glutamate and leucine rich protein 1

5 of 14

609455

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0053217

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Major pathway of rRNA processing in the nucleolus and cytosol

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47

Cytogenetic map

80D2-80D2

Sequence location

3L:23,126,025..23,132,209 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

80D2-80D2

Limits computationally determined from genome sequence between P{lacW}l(3)j1B10^j1B10 and P{lacW}l(3)j2C4^j2C4 80D3

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-47

(FlyBase, 2016)

3-44.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

43167

y¹ v¹; P{TRiP.GL01509}attP2

58267

y¹ v¹; P{TRiP.HMJ22343}attP40/CyO

82835

y¹ sc^* v¹ sev²¹; P{TKO.GS04422}attP40

43315

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02699}attP40

37011

y¹ w^*; Mi{MIC}CG33217^MI03360/TM3, Sb¹ Ser¹

66775

y¹ w^*; Mi{PT-GFSTF.2}CG33217^{MI03360-GFSTF.2}/TM6C, Sb¹ Tb¹

76643

y¹ w^*; Mi{Trojan-GAL4.2}CG33217^{MI03360-TG4.2}/TM3, Sb¹ Ser¹

NIG-Fly

HMJ22343

y¹ v¹; P{TRiP.HMJ22343}attP40/CyO

HMS02699

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02699}attP40

VDRC

v103309

P{KK112843}VIE-260B

v47818

w¹¹¹⁸; P{GD16022}v47818

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (49)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE15216

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0053217

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0053217

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Candidate stable intronic sequence RNA (sisRNA) identified within CDS of this gene.

(Pek et al., 2015)

New annotation (CG33217) in release 3.2 of the genome annotation.

(FlyBase Genome Annotators, 2004)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG33217 BcDNA:RE15216

(Rana, 2004.7.2)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

BcDNA:RE15216

CG33217

(Syrzycka et al., 2019, Transgenic RNAi Project members, 2017-, Çiçek et al., 2016, Gene Disruption Project members, 2016-, Gene Disruption Project members, 2015-, Pek et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5)

Name Synonyms

Secondary FlyBase IDs

FBan0033217
FBgn0063058

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 29 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

2768951

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q75G43

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q75G43

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0053217

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

2768951/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0053217

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0053217

FlyMine - An integrated database for Drosophila genomics

FBgn0053217

InterPro - A database of protein families, domains and functional sites

Armadillo-type fold

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG33217

MARRVEL_MODEL - MARRVEL (model organism gene)

2768951

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0053217

DroID - A comprehensive database of gene and protein interactions.

FBgn0053217

DRSC - Results frm RNAi screens

FBgn0053217

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG33217_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0053217

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

DMEL:FBGN0053217

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33217

Flygut - An atlas of the Drosophila adult midgut

FBgn0053217

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0053217

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Major pathway of rRNA processing in the nucleolus and cytosol

References (28)

The following external sites may use different assemblies or annotations than FlyBase.

-0.42

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