FlyBase Gene Report: Dmel\RYa

General Information

Symbol

Dmel\RYa

Species

D. melanogaster

Name

RYamide

Annotation Symbol

CG40733

Feature Type

protein_coding_gene

FlyBase ID

FBgn0085512

Gene Model Status

Current

Stock Availability

2 publicly available

Gene Summary

Neuropeptides RYamide-1 and RYamide-2 are ligands for the G-protein coupled receptor RYa-R (PubMed:21704020, PubMed:21843505). May suppress feeding behavior (PubMed:21704020). (UniProt, G5CKU5)

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All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

41A1-41A1

Sequence location

2R:3,647,346..3,647,765 [+]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:3647346..3647765

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

NCBI

UCSC

Ensembl

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables receptor ligand activity

inferred from physical interaction with FLYBASE:RYa-R; FB:FBgn0004842

(Ida et al., 2011)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in G protein-coupled receptor signaling pathway

inferred from direct assay

(Ida et al., 2011)

involved_in negative regulation of feeding behavior

inferred from direct assay

(Ida et al., 2011)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in extracellular region

inferred by curator from GO:0048018

(Ida et al., 2011)

located_in extracellular space

inferred by curator from GO:0048018

(Ida et al., 2011)

Gene Group (FlyBase)

NEUROPEPTIDES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

NEUROPEPTIDES -: Neuropeptides are secreted into the extracellular space where they interact with cell surface receptors (usually G protein coupled receptors). They are extremely diverse, acting as neurotransmitters, neuromodulators, hormones or growth factors. (Adapted from FBrf0211443 and PMID:27813667).

Protein Function (UniProtKB)

Neuropeptides RYamide-1 and RYamide-2 are ligands for the G-protein coupled receptor RYa-R (PubMed:21704020, PubMed:21843505). May suppress feeding behavior (PubMed:21704020).

(UniProt, G5CKU5)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RYa for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry G5CKU5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RYa-RC

FBtr0310523

NM_001110912

420

109

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

RYa-PC

FBpp0302660

13.2

109

11.43

G5CKU5

NP_001104382

EDP28140

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

G5CKU5

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RYa using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult rectal papilla

(Veenstra and Khammassi, 2017)

Additional Descriptive Data

Antibody to RYa protein labels abdominal neurons that innervate the rectal papilla and the rectal papillae.

(Veenstra and Khammassi, 2017)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\RYa in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RYa

View exonic expression by tissue for Dmel\RYa

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

5740597/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0085512

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0085512

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RYa

Transgenic constructs containing regulatory region of RYa

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)247

(Coulthard et al., 2010)

Gene not disrupted in

Df(2R)34-2

(Coulthard et al., 2010)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Other Phenotypes

Allele

abnormal feeding behavior | adult stage, with Scer\GAL4^Act5C.PI

RYa^RNAi.UAS.cUa

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP006765

3 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:RYa. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RYa - RYa-R

ion exchange chromatography, molecular sieving, Identification by mass spectrometry, inferred by author

(Ida et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0085512

MIST (protein-protein) - An integrated Molecular Interaction Database

5740597

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

(Drosophila Heterochromatin Genome Project, 2007)

protein_coding_gene

(Drosophila Heterochromatin Genome Project, 2007)

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

41A1-41A1

Sequence location

2R:3,647,346..3,647,765 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

41A1-41A1

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-55.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

55957

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04246}attP40

VDRC

v109267

P{KK116266}VIE-260B

Genomic Clones (4)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (0)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0085512

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Veenstra and Khammassi, 2017)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

New annotation (CG40733) in release 5.2 of the genome annotation.

(Drosophila Heterochromatin Genome Project, 2007)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: RYamide CG40733

(Ida et al., 2011)

Source for identity of: RYa RYamide

(FlyBase, 2009-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG40733

(Mao et al., 2024, Caizzi et al., 2016, Walls et al., 2013, Collin et al., 2011, Ida et al., 2011, Coulthard et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Drosophila Heterochromatin Genome Project, 2007)

RYa

(Lee et al., 2025, Nässel, 2025, Kubrak et al., 2022, Nässel and Zandawala, 2020)

RYamide

dRYamide

(Ida et al., 2011)

Name Synonyms

RYamide

(Veenstra and Khammassi, 2017, Ou et al., 2016, Veenstra and Ida, 2014, Caers et al., 2012)

Secondary FlyBase IDs

FBan0040733

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 19 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

5740597

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AJ784381

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

EDP28140

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

G5CKU5

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

G5CKU5

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

G5CKU5

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

5740597/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0085512

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0085512

FlyMine - An integrated database for Drosophila genomics

FBgn0085512

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG40733

MARRVEL_MODEL - MARRVEL (model organism gene)

5740597

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0085512

DRSC - Results frm RNAi screens

FBgn0085512

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0085512

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008650

MIST (protein-protein) - An integrated Molecular Interaction Database

5740597

References (24)

The following external sites may use different assemblies or annotations than FlyBase.

NA

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