FlyBase Gene Report: Dmel\CG46301

General Information

Symbol

Dmel\CG46301

Species

D. melanogaster

Name

Annotation Symbol

CG46301

Feature Type

protein_coding_gene

FlyBase ID

FBgn0283651

Gene Model Status

Current

Stock Availability

20 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

BG:DS01759.1 , BG:DS01523.1

Key Links

Genomic Location

Cytogenetic map

34F4-35A1

Sequence location

2L:14,104,090..14,160,587 [+]

Recombination map

2-50

RefSeq locus

NT_033779 REGION:14104090..14160587

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (1 term)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in nucleoplasm

inferred from sequence or structural similarity with UniProtKB:Q96MY1

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG46301 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A1W5PXH3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.44

Gene model includes transcripts encoding non-overlapping portions of the full CDS.

Gene model reviewed during 6.10

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG46301-RC

FBtr0445731

NM_001347776

2385

623

CG46301-RD

FBtr0445729

NM_135859

4048

636

CG46301-RE

FBtr0445730

NM_001347777

5632

1237

CG46301-RF

FBtr0445732

NM_001347778

2458

667

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG46301-PC

FBpp0401606

67.8

623

9.80

NP_001334738

API64989

CG46301-PD

FBpp0401604

64.0

636

6.19

NP_609703

API64987

CG46301-PE

FBpp0401605

129.2

1237

8.24

NP_001334737

API64988

CG46301-PF

FBpp0401607

72.7

667

9.43

M9PCX5

NP_001334739

API64990

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG46301 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG17341_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG46301 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG46301

View exonic expression by tissue for Dmel\CG46301

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34829/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0283651

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0283651

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG46301

Transgenic constructs containing regulatory region of CG46301

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel8032

(Ryder, 2004.4.26)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG46301^GD9223

viable, with Scer\GAL4^Mef2.PR

CG46301^GD9223

viable, with Scer\GAL4^pnr-MD237

CG46301^GD9223

viable, with Scer\GAL4^tin.CΔ4

CG46301^GD9223

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (3)

Hsap\NOL4

9 of 14

Yes

Hsap\NOL4L

5 of 14

Yes

Hsap\ZBED9

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Nol4

8 of 14

Yes

Rnor\Nol4l

5 of 14

Yes

Mus musculus (laboratory mouse) (2)

Mmus\Nol4

8 of 14

Yes

Mmus\Nol4l

7 of 14

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\nol4

8 of 13

Yes

Xtro\nol4l

5 of 13

Yes

Danio rerio (Zebrafish) (2)

Drer\nol4la

8 of 14

Yes

Drer\nol4lb

6 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP008187

4 of 12

Yes

Agam\AgaP_AGAP008189

3 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG46301. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NOL4; nucleolar protein 4

9 of 14

603577

NOL4L; nucleolar protein 4 like

5 of 14

618893

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-50

Cytogenetic map

34F4-35A1

Sequence location

2L:14,104,090..14,160,587 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34F4-35A1

Limits computationally determined from genome sequence between P{lacW}l(2)34Fa^k00811&P{PZ}wb⁰⁹⁴³⁷ and P{EP}elB^EP2039&P{PZ}osp^rJ571

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-50

(FlyBase, 2016)

2-45.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (20)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

600944

w¹¹¹⁸; P{VT007193-p65.AD}attP40

74000

w¹¹¹⁸; P{VT007193-GAL4.DBD}attP2

75165

w¹¹¹⁸; P{VT007194-GAL4.DBD}attP2

43070

y¹ w^*; Mi{MIC}Dsff1^MI06486

56654

y¹ w^*; Mi{MIC}CG46301^MI12094

Korea

12581

P{VT007193-GAL4}attP2

13373

P{VT007195-GAL4}attP2

14840

P{VT007194-GAL4}attP2

14719

P{VT007193-lexA::GADfl}attP40

16879R-1

v102380

v32761

w¹¹¹⁸; P{GD9223}v32761

More stocks available...

Genomic Clones (49)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (15)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP31136

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0283651

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of:CG16879 CG17341

(FlyBase Genome Annotators, 2016)

Additional comments

Annotations CG16879 and CG17341 merged as CG46301 in release 6.10 of the genome annotation; supported by RNA-Seq junction data and alignment to similar proteins in other insect species.

(FlyBase Genome Annotators, 2016)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

BG:DS01523.1

(Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12)

BG:DS01759.1

(Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12)

CG16879

(National Institute of Genetics Fly Stocks, 2022-, Japanese National Institute of Genetics, 2012.5.21, Keleman et al., 2009.8.5, Bhutkar et al., 2008)

CG17341

(National Institute of Genetics Fly Stocks, 2022-, Hazelett et al., 2012, Bhutkar et al., 2008)

CG46301

(Allen et al., 2026, National Institute of Genetics Fly Stocks, 2022-, Meltzer et al., 2019, FlyBase Genome Annotators, 2016, Gene Disruption Project members, 2016-)

DS01759.1

(Reese et al., 2000)

Name Synonyms

Secondary FlyBase IDs

FBgn0028904
FBgn0028906

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34829

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A1W5PXH3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A1W5PXH3

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34829/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0283651

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0283651

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG46301

MARRVEL_MODEL - MARRVEL (model organism gene)

34829

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0283651

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG17341_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0283651

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031212

References (35)

The following external sites may use different assemblies or annotations than FlyBase.

NA

Report Sections