FB2026_03 , released September 17, 2026
Human Disease Model Report: alcohol, response to, Dmelapt-related
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General Information
Name
alcohol, response to, Dmel\apt-related
FlyBase ID
FBhh0001119
Disease Ontology Term
Parent Disease
OMIM
Overview

In a genetic screen for Drosophila mutants with altered sensitivity to ethanol-induced sedation, mutations in the Dmel\apt gene were recovered. No genes orthologous to Dmel\apt have been identified in human. Loss-of-function mutations, RNAi targeting constructs, and alleles caused by insertional mutagenesis have been generated for Dmel\apt.

Loss-of-function mutations of Dmel\apt exhibit decreased ethanol sedation sensitivity; neuronal expression of apt is required for normal ethanol sedation sensitivity. To define specific neurons in which apt function is required to regulate this function, a collection of GAL4 lines expressed in the brain were used to reduce apt expression via RNAi in small sets of neurons within the brain. Ethanol sedation sensitivity is dramatically reduced using the neuropeptidergic driver corazonin-GAL4 (Crz-GAL4) in this assay. It is proposed that apt regulates the activity of Crz neurons and/or release of the neuropeptide during ethanol exposure. (See human disease model report 'alcohol, response to, Crz neuropeptide-related' FBhh0001120).

[updated Oct. 2019 by FlyBase; FBrf0222196]

Disease Summary Information
Parent Disease Summary: alcohol use disorder, susceptibility to (fly models overview)
Symptoms and phenotype

Alcoholism can be defined as persistence of excessive drinking over a long period of time despite adverse health effects and disruption of social relations (Morozova et al., 2014; pubmed:24395673).

(FlyBase Curators, 2013-)

The 2013 Diagnostic and Statistical Manual of Mental Disorders (DSM) combined the two former categorizations of abnormal alcohol use (alcohol abuse and alcohol dependence) into one diagnosis: alcohol use disorder. The severity of an individual's AUD is broken into classifications: mild, moderate, or severe. "Alcoholism" is a non-medical term often used to describe a severe form of alcohol use disorder. (https://www.therecoveryvillage.com/recovery-blog/alcoholism-alcohol-use-disorder-whats-difference/)

(FlyBase Curators, 2013-)

Excessive alcohol consumption is associated with increased risk of different types of cancer, higher cardiovascular disease mortality, birth defects, liver diseases, and neuropsychiatric disorders (Morozova et al., 2014; pubmed:24395673).

(FlyBase Curators, 2013-)

Alcoholism is a multifactorial, genetically influenced disorder. [from MIM:103780; 2017.12.19]

(OMIM, 2013-)
Specific Disease Summary: alcohol, response to, Dmel\apt-related
OMIM report
Human gene(s) implicated
Symptoms and phenotype
Genetics
Cellular phenotype and pathology
Molecular information

Dmel\apontic (apt) is a SANT-MYB domain-containing transcription factor.

(FlyBase Curators, 2013-)
External links
    Disease synonyms
    Ortholog Information
    Human gene(s) in FlyBase
      Other mammalian ortholog(s) used
        D. melanogaster Gene Information (1)
        Dmel\apt
        Gene Snapshot
        apontic (apt) encodes a DNA-binding protein that acts as a transcriptional regulator and can function in translational repression. It plays roles in cell migration, cell fate specification, and morphogenesis via STAT signaling pathway inhibition. [Date last reviewed: 2019-03-07]
        Molecular function (GO)
        Cellular component (GO)
        Gene Groups / Pathways
        Comments on ortholog(s)

        No human ortholog(s) identified.

        Orthologs and Alignments from DRSC
        DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans
        H. sapiens (Human)
        Other Genes Used: Viral, Bacterial, Synthetic (0)
          Summary of Physical Interactions (13 groups)
          apt
          protein-protein
          Interacting group
          Assay
          References
          apt - abd-A
          bimolecular fluorescence complementation, fluorescence microscopy
          (Bischof et al., 2018, Baëza et al., 2015)
          apt - Abd-B
          bimolecular fluorescence complementation, fluorescence microscopy
          (Baëza et al., 2015)
          apt - Antp
          bimolecular fluorescence complementation, fluorescence microscopy
          (Baëza et al., 2015)
          apt - bru1
          anti bait coimmunoprecipitation, western blot
          (Lie and Macdonald, 1999)
          apt - Cenp-C
          anti tag coimmunoprecipitation, peptide massfingerprinting
          (Lipinszki et al., 2015)
          apt - cnn
          anti bait coimmunoprecipitation, peptide massfingerprinting
          (Habermann et al., 2012)
          apt - Hr78
          two hybrid array
          (Shokri et al., 2019)
          apt - mbf1
          anti tag coimmunoprecipitation, edman degradation, pull down, western blot, anti bait coimmunoprecipitation
          (Liu et al., 2003)
          apt - Scr
          bimolecular fluorescence complementation, fluorescence microscopy
          (Baëza et al., 2015)
          apt - Tbp
          anti bait coimmunoprecipitation, western blot
          (Liu et al., 2003)
          apt - Ubx
          bimolecular fluorescence complementation, fluorescence microscopy, two hybrid
          (Bischof et al., 2018, Baëza et al., 2015, Hsiao et al., 2014)
          RNA-protein
          Interacting group
          Assay
          References
          apt - Fmr1
          anti bait coimmunoprecipitation, quantitative reverse transcription pcr, clip-seq
          (Buddika et al., 2021)
          apt - osk
          nucleic acid uv cross-linking assay, autoradiography
          (Lie and Macdonald, 1999)
          Alleles Reported to Model Human Disease (Disease Ontology) (2 alleles)
          apt
          Models Based on Experimental Evidence ( 0 )
          Allele
          Disease
          Evidence
          References
          Modifiers Based on Experimental Evidence ( 2 )
          Allele
          Disease
          Interaction
          References
          aptUAS.cEa
          ameliorates  carcinoma
          modeled by scribRNAi.UAS.cUa
          (Song et al., 2023)
          aptGD1367
          exacerbates  carcinoma
          modeled by scribRNAi.UAS.cUa
          (Song et al., 2023)
          Alleles Representing Disease-Implicated Variants
          Genetic Tools, Stocks and Reagents
          Sources of Stocks
          Contact lab of origin for a reagent not available from a public stock center.
          Bloomington Stock Center Disease Page
          Related mammalian, viral, bacterial, or synthetic transgenes
          Allele
          Transgene
          Publicly Available Stocks
          Selected Drosophila transgenes
          Allele
          Transgene
          Publicly Available Stocks
          aptUAS.cEa
          P{UAS-apt.E}
          RNAi constructs available
          Allele
          Transgene
          Publicly Available Stocks
          aptGD1367
          P{GD1367}
          w1118; P{GD1367}v4289
          aptJF02134
          P{TRiP.JF02134}
          y1 v1; P{TRiP.JF02134}attP2
          aptKK112298
          P{KK112298}
          P{KK112298}VIE-260B
          Selected Drosophila classical alleles
          Allele
          Allele class
          Mutagen
          Publicly Available Stocks
          aptMT287
          loss of function allele
          Delta2-3 transposase
          References (3)