FlyBase Allele Report: Dmel\apt[GD1367]

General Information

Symbol

Dmel\apt^GD1367

Species

D. melanogaster

Name

FlyBase ID

FBal0209069

Feature type

allele

Associated gene

Dmel\apt

Associated Insertion(s)

Carried in Construct

P{GD1367}

Also Known As

UAS-apt^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1367}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1367

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by scrib^RNAi.UAS.cUa

(Song et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Bx-MS1096

(Wang et al., 2017)

wing disc, with Scer\GAL4^ap-md544

(Wang et al., 2017)

Detailed Description

Statement

Reference

Expression of apt^GD1367 under the control of Scer\GAL4^Bx-MS1096 leads to a decrease in the size of the adult wing, and reduces the area between vein L3 and vein L4 as a percentage of the whole wing area, as compared to controls.

Wing discs expressing apt^GD1367 under the control of Scer\GAL4^ap-md544 exhibit growth disadvantage as evidenced by a significant decrease in size as compared to controls.

(Wang et al., 2017)

Flies expressing apt^GD1367 under the control of Scer\GAL4^elav-C155 (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi efficiency) show a significant reduction in ethanol sedation sensitivity compared to wild type. Using the temperature sensitive Scer\GAL80^ts.αTub84B allele to restrict expression to a specific stage demonstrates that a decrease in ethanol sedation sensitivity is seen both when expression is restricted to metamorphosis and also when it is restricted to days 3-7 of adulthood.

Flies expressing apt^GD1367 under the control of Scer\GAL4^Crz.PU (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi efficiency) show a significant reduction in ethanol sedation sensitivity compared to wild type.

(McClure and Heberlein, 2013)

Adults expressing apt^GD1367 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 80-90% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1367

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

apt^GD1367/Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, smo^PKA12.UAS

(Wang et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

apt^GD1367/Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, smo^PKA12.UAS

(Wang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of apt^GD1367 enhances the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096.

(Wang et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v4289

w¹¹¹⁸; P{GD1367}v4289

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1367

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

apt^GD1367

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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