FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Map205, polo
FlyBase ID
FBig0000029035
Interaction Network
Interactions Browser links
Map205 network
polo network
Reported Interactions
FBrf0206217-1.coIP.MS
Reference
Archambault et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
polo 

Polo

bait

PrA tag

polo 
Map205 

Map205

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
polo 
PB1 domain residue 396
mutation disrupting interaction
aa 396

V396A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Archambault et al., 2008)
FBrf0206217-2.coIP.MS
Reference
Archambault et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Map205 

Map205

bait

PrA tag

polo 

Polo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Archambault et al., 2008)
FBrf0206217-3.coIP.WB
Reference
Archambault et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Map205 

Map205

bait

PrA tag

Map205 
Map205 
polo 

Polo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Map205 
interaction domain
sufficient binding region
aa 254-416

coordinates relative to Map205-PA

Map205 
interaction domain residue 283
mutation disrupting interaction
aa 283

S283D or S283E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct or endogenous gene; prey produced from endogenous gene.

(Archambault et al., 2008)
FBrf0206217-4.coIP.WB
Reference
Archambault et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Map205 

Map205

bait
polo 

Polo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Archambault et al., 2008)
FBrf0206217-5.PD.WB
Reference
Archambault et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Map205 

Map205

bait

GST tag

Map205 
Map205 
polo 

Polo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Map205 
interaction domain
sufficient binding region
aa 254-416

coordinates relative to Map205-PA

Map205 
interaction domain residue 283
mutation decreasing interaction
aa 283

S283D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Archambault et al., 2008)

Phosphorylation of Map205 with human CycB-Cdk1 decreases the polo-Map205 interaction.

(Archambault et al., 2008)
FBrf0218395-8274.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Map205 
unspecified role
Map205 
unspecified role
Map205 
unspecified role
polo 
unspecified role
polo 
unspecified role
polo 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 95.31741

FBrf0226557-1.coIP.WB
Reference
Kachaner et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Map205 

Map205

bait

PtA tag

polo 

polo

prey

Myc tag

polo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
polo 
phosphorylation site
mutation decreasing interaction
aa 182

T182D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kachaner et al., 2014)

Coexpression of aurB/phosphorylation of polo decreases interaction. Interaction destabilized by treatment with polo inhibitor BI 2536.

(Kachaner et al., 2014)
FBrf0226557-2.PD.WB
Reference
Kachaner et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Map205 

Map205

bait

GST tag

Map205 
polo 

polo

prey
polo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Map205 
interaction domain
sufficient binding region
aa 254-375
polo 
phosphorylation site
mutation decreasing interaction
aa 182

T182D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction destabilized by treatment with polo inhibitor BI 2536.

(Kachaner et al., 2014)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from cell extract of S2 cell line.

(Kachaner et al., 2014)
FBrf0237276-2.coIP.WB
Reference
Kachaner et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Map205 

Map205

bait

PrA tag

polo 

polo

prey

GFP tag

polo 
polo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
polo 
kinase domain residue 182
mutation decreasing interaction
aa 182

T182D

polo 
NLS domain
mutation decreasing interaction
aa 106-118

K106A,K107A,R108A,K115A,R116A,R117A,K118A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kachaner et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)