Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Bait was affinity purified by virtue of the His tag, and proteins were further purified by an anti-phosphotyrosine resin.

Interaction in vitro; bait derived from S2 cell extract; prey produced as a recombinant fusion protein in bacterial system and labeled in vitro.

dock immunoprecipitated protein from S2 cell extract was separated by SDS-PAGE and transferred to nitrocellulose. The blot was incubated with [32]P-labelled dock. The dock-interacting 270kDa band from S2 extracts disappeared upon Dscam1-directed RNAi.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The first SH3 domain of dock binds preferentially to the first Dscam1 PXXP motif, and the third SH3 domain of dock binds preferentially to the second Dscam1 PXXP motif.

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The first SH3 domain of dock binds preferentially to the first Dscam1 PXXP motif, and the third SH3 domain of dock binds preferentially to the second Dscam1 PXXP motif. The second dock SH3 domain binds to the C-terminal polyproline sequence (no PXXP motifs present).

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Proteins identified with Mascot protein scores >100 that were undetected in control samples were considered specific Dscam binding partners.

Source was larvae of engineered fly line; bait produced from tagged endogenous gene; prey produced from endogenous gene.

The Dscam1-dock interaction is stronger with the TM2 Dscam1 isoform than the TM1 isoform.

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

The Dscam1-dock interaction is stronger with the TM2 Dscam1 isoform than the TM1 isoform.

Dscam1 isoforms TM1 and TM2 interact with dock equally well in S2 cells.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.