twin and Pop2 copurify in DEAE fractionation and subsequent gel filtration as part of the CCR4-NOT complex.

twin and Pop2 containing fractions contain poly(A)-specific 3\' exonuclease activity.

Source was cytoplasmic extract of S2 cell line; proteins produced from endogenous genes.

Cytoplasmic extract was first DEAE fractionated, then separated by gel filtration.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Catalytically inactive Pop2 is able to bind twin.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Catalytically inactive twin mutant proteins are able to bind Pop2.

Experiments were done in bam[Δ86] mutant ovaries, which contain only undifferentiated pre-cystoblasts.

Source was adult female ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

The twin-Not1 interaction is not affected by RNase treatment.

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.

Hexameric CCR4-NOT-mini complex captured as 15 pairwise interactions.

Source was live S2R+ cells; bait and prey produced from endogenous genes.

Pop2 was fused to a plasma membrane or ER membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.

twin was fused to a plasma membrane or ER membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.

Source was live S2R+ cells; bait and prey produced from endogenous genes.