Bait endogenous to adult extract.

Prey endogenous to adult extract.

The 5MER snf mutation, which changes residues 41-45 from VALKT to U1A-like LVSRS, abolishes the interaction of full length snf and U2A proteins.

The E21D snf mutation in the N-terminal RRM motif does not affect snf interaction with U2A protein.

Both the N-terminal and C-terminal RRM motifs of snf can interact with U2A protein.

The 5MER snf mutation, which changes residues 41-45 from VALKT to U1A-like LVSRS, abolishes the interaction of full length snf and U2A proteins.

The E21D snf mutation has only a weak effect on the interaction of full length snf with U2A. However, in the context of a truncated snf protein lacking the C-terminal RRM motif, the E21D snf mutation does abolish the interaction with U2A.

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Positive control.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

Structures of snf-U2A binary complex as well as of snf-U2A-U1 snRNA and snf-U2A-U2 snRNA ternary complexes.

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

Kd = 4.2nM