FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hakai, fl(2)d
FlyBase ID
FBig0000039843
Interaction Network
Interactions Browser links
Hakai network
fl(2)d network
Reported Interactions
FBrf0218395-1744.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fl(2)d 
unspecified role
fl(2)d 
unspecified role
fl(2)d 
unspecified role
Hakai 
unspecified role
Hakai 
unspecified role
Hakai 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 67.70343

FBrf0248701-4.coIP.WB
Reference
Wang et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hakai 

Hakai

bait

GFP tag

fl(2)d 

Fl(2)d

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2021)
FBrf0248701-6.coIP.WB
Reference
Wang et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hakai 

Hakai

bait

GFP tag

fl(2)d 

Fl(2)d

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Wang et al., 2021)

Both short and long isoforms of Hakai interact with fl(2)d.

(Wang et al., 2021)
FBrf0249274-1.coIP.WB
Reference
Bawankar et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Hakai 

Hakai

bait

Myc tag

Hakai 
fl(2)d 

Fl(2)d

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hakai 
RING domain
mutation decreasing interaction
aa 180,183,196,198,201,204,213,216

C180A,C183A,C196A,H198A,C201A,C204A,C213A,C216A, coordinates relative to Hakai-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bawankar et al., 2021)

The 473aa isoform of Hakai, corresponding to Hakai-PF, was used. Knockdown of vir reduces the Hakai-fl(2)d interaction.

(Bawankar et al., 2021)
FBrf0249274-15.coIP.MS
Reference
Bawankar et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Hakai 

Hakai

bait

Myc tag

fl(2)d 

Fl(2)d

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Bawankar et al., 2021)

The 473aa isoform of Hakai, corresponding to Hakai-PF, was used.

(Bawankar et al., 2021)
FBrf0249274-4.Y2H
Reference
Bawankar et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fl(2)d 

Fl(2)d

bait

fused to GAL4 DNA-binding domain

Hakai 

Hakai

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bawankar et al., 2021)

Two-hybrid system: yeast GAL4-BD/GAL4-AD. The 473aa isoform of Hakai, corresponding to Hakai-PF, was used.

(Bawankar et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)