Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Overall SAINT probability of interaction = 1.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Overall SAINT probability of interaction = 1.
Purified ave-cnk heterodimer was mixed with an equimolar ratio of ksr-Dsor1 heterodimer, which eluted as a higher order complex as series of binary interactions that do not necessarily indicate direct protein-protein contact. Mutations in cnk or ksr interfere with the higher order cnk-ave-ksr-Dsor1 complex.
Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.
within the context of 654-966; M903D; coordinates relative to ksr-PA
within the context of 654-966; W896R; coordinates relative to ksr-PA
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; proteins produced as recombinant fusion proteins in a baculovirus and insect cell system.