FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
par-6, aPKC
FlyBase ID
FBig0000058573
Interaction Network
Interactions Browser links
par-6 network
aPKC network
Reported Interactions
FBrf0157155-12.PD.A
Reference
Betschinger et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GST tag

par-6 
aPKC 

aPKC

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
par-6 
N-terminal region
necessary binding region
aa 1-121
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Betschinger et al., 2003)
FBrf0157155-2.coIP.MS
Reference
Betschinger et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 6embryonic stage 11embryonic stage 9early extended germ band stageembryonic stage 8embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait
aPKC 

aPKC

prey

47% coverage

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Betschinger et al., 2003)
FBrf0157155-6.coIP.WB
Reference
Betschinger et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 6embryonic stage 11embryonic stage 9early extended germ band stageembryonic stage 8embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Betschinger et al., 2003)
FBrf0157155-7.PD.WB
Reference
Betschinger et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 6embryonic stage 11embryonic stage 9early extended germ band stageembryonic stage 8embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GST tag

aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Betschinger et al., 2003)
FBrf0179253-3.PD.A
Reference
Hutterer et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GST tag

aPKC 

aPKC

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Hutterer et al., 2004)
FBrf0179253-5.coIP.WB
Reference
Hutterer et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Hutterer et al., 2004)
FBrf0179422-3.coIP.WB
Reference
Ruiz-Canada et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Presynaptic over-expression of a constitutively active form of aPKC increases interaction with par-6.

(Ruiz-Canada et al., 2004)

Source was third instar larval body walls of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ruiz-Canada et al., 2004)
FBrf0187224-4.Y2H
Reference
Djiane et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aPKC 

aPKC

bait

fused to DNA-binding domain

par-6 

Par6

prey

fused to activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Djiane et al., 2005)

aPKC interacts with par-6, but not with Patj, sdt or dlg1 in this assay.

(Djiane et al., 2005)

Two-hybrid system: yeast

(Djiane et al., 2005)

Positive control.

(Djiane et al., 2005)
FBrf0206088-1.coIP.WB
Reference
Wirtz-Peitz et al., 2008
Description
physical association
Assay
Collection
Source/Stage
larval brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brains of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wirtz-Peitz et al., 2008)
FBrf0206088-4.PD.WB
Reference
Wirtz-Peitz et al., 2008
Description
physical association
Assay
Collection
Source/Stage
larval brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GST tag

par-6 
par-6 
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
par-6 
phosphorylation site
mutation disrupting interaction
aa 34

S34D or S34E

par-6 
PB1 domain
mutation disrupting interaction
aa 23

K23A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type larval brain extract.

(Wirtz-Peitz et al., 2008)

Phosphorylation of GST-par-6 by aur inhibits the par-6-aPKC interaction. Similarly, phosphomimetic forms of par-6 do not pull down endogenous aPKC.

(Wirtz-Peitz et al., 2008)
FBrf0206088-9.PD.WB
Reference
Wirtz-Peitz et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GST tag

aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryo extract.

(Wirtz-Peitz et al., 2008)
FBrf0208055-3.coIP.WB
Reference
Atwood and Prehoda, 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Atwood and Prehoda, 2009)

pins does not interact with aPKC in this experiment.

(Atwood and Prehoda, 2009)
FBrf0209133-1.coIP.WB
Reference
Kim et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
par-6 

PAR-6

bait
aPKC 

aPKC

prey

GFP tag

aPKC 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aPKC 
PB1 domain residue C122
mutation disrupting interaction
aa 122

C122Y, "psu69" mutation, coordinates relative to aPKC-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Kim et al., 2009)
FBrf0209133-2.coIP.WB
Reference
Kim et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
aPKC 

aPKC

bait

GFP tag

aPKC 
par-6 

PAR-6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aPKC 
PB1 domain residue C122
mutation disrupting interaction
aa 122

C122Y, "psu69" mutation, coordinates relative to aPKC-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kim et al., 2009)
FBrf0217811-1.coIP.WB
Reference
Neumüller et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GFP tag

aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a GFP-tagged par-6 construct in a parδ226 mutant background.

(Neumüller et al., 2012)

Prey endogenous to embryonic extract.

(Neumüller et al., 2012)
FBrf0217811-3.coIP.MS
Reference
Neumüller et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GFP tag

aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a GFP-tagged par-6 construct in a parδ226 mutant background.

(Neumüller et al., 2012)

Prey endogenous to embryonic extract.

(Neumüller et al., 2012)
FBrf0218395-2936.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aPKC 
unspecified role
aPKC 
unspecified role
aPKC 
unspecified role
par-6 
unspecified role
par-6 
unspecified role
par-6 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 215.32307

FBrf0233695-3.coIP.MS
Reference
Calero-Cuenca et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aPKC 

aPKC

bait

Tetra tag

par-6 

Par6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Calero-Cuenca et al., 2016)
FBrf0243203-3.coIP.WB
Reference
Nunes de Almeida et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par6

bait

FLAG tag

par-6 
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
par-6 
PB1 domain residue 23
mutation disrupting interaction
aa 23

K23A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Nunes de Almeida et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (11)