FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Charon, Rel
FlyBase ID
FBig0000077614
Interaction Network
Interactions Browser links
Charon network
Rel network
Reported Interactions
FBrf0218395-2938.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rel 
unspecified role
Rel 
unspecified role
Rel 
unspecified role
Charon 
unspecified role
Charon 
unspecified role
Charon 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 89.53358

FBrf0227292-232.TFIN
Reference
Rhee et al., 2014
Description
physical association
Assay
Collection
TF_Interaction_Network-2
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Rel 

FBgn0014018

unspecified role
Rel 

FBgn0014018

unspecified role
Rel 

FBgn0014018

unspecified role
Charon 

FBgn0031317

unspecified role
Charon 

FBgn0031317

unspecified role
Charon 

FBgn0031317

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

(Rhee et al., 2014)

HGScore = 46.412452

(Rhee et al., 2014)
FBrf0233348-2.coIP.WB
Reference
Ji et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Charon 

Charon

bait

GFP tag

Rel 

Relish

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Ji et al., 2016)

Charon interacts with both full length Rel and, upon infection, with the Rel68 cleaved fragment.

(Ji et al., 2016)
FBrf0233452-1.coIP.WB
Reference
Morris et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Charon 

Pickle

bait

FLAG tag

Rel 

Relish

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2* cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Morris et al., 2016)
FBrf0233452-2.coIP.WB
Reference
Morris et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Charon 

Pickle

bait

V5 tag

Rel 

Relish

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Charon binds Rel but not other NF-kB members dl or Dif.

(Morris et al., 2016)

Source was cell extract of S2* cell line; bait produced from transfected construct; prey produced from transfected construct.

(Morris et al., 2016)
FBrf0233452-3.coIP.WB
Reference
Morris et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Charon 

Pickle

bait

FLAG tag

Charon 
Rel 

Relish

prey

V5 tag

Rel 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rel 
RelN portion
sufficient binding region
Charon 
C-terminal region
sufficient binding region
aa 277-524

coordinates relative to Charon-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2* cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Morris et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)