FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
fzy, BubR1
FlyBase ID
FBig0000085542
Interaction Network
Interactions Browser links
fzy network
BubR1 network
Reported Interactions
FBrf0210988-2.coIP.WB
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
fzy 

Cdc20

bait
BubR1 

BubR1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2010)

The fzy-BubR1 interaction is increased upon metaphase arrest via Cdc27 depletion.

(Li et al., 2010)
FBrf0210988-4.coIP.WB
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fzy 

Cdc20

bait
BubR1 

BubR1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2010)
FBrf0210988-6.coIP.WB
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fzy 

Cdc20

bait

GFP tag

BubR1 

BubR1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Li et al., 2010)

The fzy-BubR1 interaction is increased upon metaphase arrest via MG132 treatment.

(Li et al., 2010)
FBrf0221865-1.coIP.WB
Reference
Conde et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
fzy 

Cdc20

bait
BubR1 

BubR1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cells were treated with MG132 for one hour, then two hours with colchicine, to induce the formation of the mitotic checkpoint complex (MCC).

(Conde et al., 2013)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Conde et al., 2013)

The fzy-BubR1 interaction is not detected in cells depleted of polo activity by RNAi or the inhibitor BI2536, suggesting a role for polo in the spindle assembly checkpoint.

(Conde et al., 2013)

The fzy-BubR1 interaction is reduced in cells depleted of mad2 activity by RNAi.

(Conde et al., 2013)
FBrf0221865-6.coIP.WB
Reference
Conde et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
BubR1 

BubR1

bait
fzy 

Cdc20

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cells were treated with MG132 for one hour, then two hours with colchicine, to induce the formation of the mitotic checkpoint complex (MCC).

(Conde et al., 2013)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Conde et al., 2013)
FBrf0230940-1.PD.WB
Reference
Derive et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BubR1 

BubR1

bait

GST tag

BubR1 
fzy 

Fzy

prey

GFP tag

fzy 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
BubR1 
N-terminal region
sufficient binding region
aa 1-337
fzy 
interaction region
mutation decreasing interaction
aa 210-212

DYY to AAA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic embryonic extract.

(Derive et al., 2015)
FBrf0244511-3.coIP.WB
Reference
Cunha-Silva et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
BubR1 

BubR1

bait
fzy 

Cdc20

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Cunha-Silva et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)