FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pav, tum
FlyBase ID
FBig0000086072
Interaction Network
Interactions Browser links
pav network
tum network
Reported Interactions
FBrf0158856-4.Y2H
Reference
Somers and Saint, 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 9blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic cycle 3embryonic stage 4embryonic cycle 9embryonic stage 2embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pav 

PAV

bait
tum 

RacGAP50C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey isolated from embryonic cDNA library.

(Somers and Saint, 2003)

Two-hybrid system: yeast LexA/VP16.

(Somers and Saint, 2003)
FBrf0158856-5.Y2H
Reference
Somers and Saint, 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pav 

PAV

bait
pav 
tum 

RacGAP50C

prey
tum 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pav 
region
unspecified role
aa 1-773
tum 
extreme N-terminal region
unspecified role
aa 1-65
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey was cloned reagent (previously isolated).

(Somers and Saint, 2003)

Two-hybrid system: yeast LexA/VP16.

(Somers and Saint, 2003)
FBrf0158856-6.coIP.WB
Reference
Somers and Saint, 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 10blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic stage 9embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pav 

PAV

bait
tum 

RacGAP50C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0158856-7.coIP.WB
Reference
Somers and Saint, 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 10blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic stage 9embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tum 

RacGAP50C

bait
pav 

PAV

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0211056-1.Y2H
Reference
Jones et al., 2010
Description
physical association
Assay
Collection
Source/Stage
/ embryonic stage 1embryonic stage 9blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic cycle 3embryonic stage 4embryonic cycle 9embryonic stage 2embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tum 

Tum

bait
pav 

Pav

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey isolated from embryonic cDNA library.

(Jones et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Jones et al., 2010)
FBrf0212581-1.coIP.MS
Reference
Montembault et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
pav 

Pav-KLP

bait

PrA tag

tum 

RacGAP50C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged pav construct.

(Montembault et al., 2010)
FBrf0212581-2.coIP.MS
Reference
Montembault et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
tum 

RacGAP50C

bait

PrA tag

pav 

Pav-KLP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged tum construct.

(Montembault et al., 2010)
FBrf0214097-3.Y2H
Reference
Ebrahimi et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pav 

Pav-KLP

bait

fused to GAL4 DNA-binding domain

tum 

RacGAP

prey

fused to VP16 activation domain

tum 
tum 
tum 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tum 
Polo kinase binding region
necessary binding region
aa 149-155
tum 
Polo kinase binding region
mutation decreasing interaction
aa 119-122

multiple alanine substitutions

tum 
Polo kinase binding region
mutation disrupting interaction
aa 154-155

double alanine substitution

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Ebrahimi et al., 2010)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Ebrahimi et al., 2010)
FBrf0218395-1158.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pav 
unspecified role
pav 
unspecified role
pav 
unspecified role
tum 
unspecified role
tum 
unspecified role
tum 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 99.20520

FBrf0242950-55.PL.MS
Reference
Mannix et al., 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pav 

Pav

bait

APEX tag

tum 

tum

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Saint score = 0.94

(Mannix et al., 2019)

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Mannix et al., 2019)
FBrf0242950-67.PLA
Reference
Mannix et al., 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pav 

Pav

bait

APEX, FLAG tag

tum 

tum

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Mannix et al., 2019)
FBrf0243783-6.PD.A
Reference
Lie-Jensen et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tum 

Tum

bait

GST tag

pav 

Pav

prey

Myc tag, [35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Lie-Jensen et al., 2019)
FBrf0247253-5.coIP.WB
Reference
Sechi et al., 2020
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pav 

Pav

bait

YFP tag

tum 

RacGAP50C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was testes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sechi et al., 2020)
FBrf0256485-6.CM.WB
Reference
Davidson et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
tum 

Tum

neutral component
tum 

Tum

neutral component
tum 

Tum

neutral component
pav 

Pav

neutral component
pav 

Pav

neutral component
pav 

Pav

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear cell extract of Kc167 cell line; proteins produced from endogenous genes.

(Davidson et al., 2023)

wash, FAM21 and tum comigrate in a 900kD complex. wash, pav and tum comigrate in a 750kD complex.

(Davidson et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (9)