Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated on a glycerol gradient and fractions containing brm and osa were used for immunoprecipitation.

osa can immunoprecipitate brm and mor, but not polybromo, suggesting that osa and polybromo exist in distinct Brahma complexes.

Source was embryonic nuclear extract wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

osa is present with brm and mor in a distinct Brahma complex that lacks polybromo and Bap170.

Source was embryonic nuclear extract wild-type fly line; proteins produced from endogenous gene.

Nuclear extract was subjected to several chromatographic steps to isolate brm-containing complexes.