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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000090443
Interaction Network
Interactions Browser links
brm network
Snr1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0083116-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

brm

bait

HA tag

Snr1 

snr1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

brm and Snr1 are also present in large molecular mass complexes, as determined by gel filtration chromatography of embryonic nuclear extracts.

This interaction persists in the presence of ethidium bromide, and so is not due to indirect interactions via DNA.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0104901-6.PD.MS
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

BRM

bait

His tag

Snr1 

BAP45 (SNR1)

prey

two unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

brm, mor, dalao, Hsc70-4, Bap60, Bap55, Snr1 and Bap47 (actin) were identified as components of the Brahma complex.

Nuclear extract was fractionated through several different chromatographic steps, selecting for brm-containing fractions, before pull down of His tagged brm.

FBrf0104901-9.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

BRM

bait

HA tag

Snr1 

SNR1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0124978-18.coIP.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

BRM

bait
Snr1 

Snr1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated on a glycerol gradient and fractions containing brm and osa were used for immunoprecipitation.

FBrf0124978-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

Brm

bait
Snr1 

Snr1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

brm coimmunoprecipitates osa and Snr1 (Brahma complex), but not Iswi.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0127148-7.coIP.MW
Reference
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

BRM

bait
Snr1 

BAP45

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

brm, osa, Bap170, p400, mor, dalao, Bap60, Bap55, Snr1, Bap47 (actin) and Bap26 were identified as components of the Brahma complex.

Source was embryonic nuclear extract of wild-type fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Nuclear extract was fractionated through several different chromatographic steps, selecting for brm-containing fractions, before immunoprecipitation of brm.

FBrf0155964-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Snr1 

SNR1

bait
brm 

BRM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extracts; bait produced from endogenous gene; prey produced from endogenous gene

FBrf0155964-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Snr1 

SNR1

bait
brm 

BRM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The E1 mutant form of Snr1 carrying a G256D substitution is capable of interacting with brm.

Source was pupal extracts; bait produced from endogenous gene; prey produced from endogenous gene

FBrf0174961-8.coIP.MS
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

BRM

bait
Snr1 

Snr1/BAP45

prey

6 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

brm, mor, dalao, Bap60, Bap55, Snr1 and actin form the Brahma (BRM) complex, together with either osa (BAP BRM complex) or polybromo and Bap170 (PBAP BRM complex).

Source was embryonic nuclear extract wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated by ammonium sulfate precipitation and several chromatographic steps before being subjected to immunoprecipitation.

FBrf0180290-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Snr1 

SNR1

bait
brm 

BRM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Embryonic extracts were prepared from flies expressing MYC-tagged DmCDK2

Source was embryonic extracts; bait produced endogenous gene; prey derived from transgenic construct

FBrf0180290-6.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Snr1 

SNR1

bait

GST tag

Snr1 
brm 

BRM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Snr1 
interaction domain
necessary binding region
aa 14-370

coordinates relative to Snr1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from endogenous gene.

Embryonic extracts were prepared from flies expressing MYC-tagged DmCDK2

FBrf0218395-4200.DPiM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 
unspecified role
brm 
unspecified role
brm 
unspecified role
Snr1 
unspecified role
Snr1 
unspecified role
Snr1 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 107.89550

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)