FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000092600
Interaction Network
Interactions Browser links
Panx network
nxf2 network
Reported Interactions
FBrf0218395-4708.DPiM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 
unspecified role
Panx 
unspecified role
Panx 
unspecified role
nxf2 
unspecified role
nxf2 
unspecified role
nxf2 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 64.39979

FBrf0243113-1.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panx

bait

GFP tag

nxf2 

Nxf2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0243113-3.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

bait

GFP tag

Panx 

Panx

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0243113-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

bait

FLAG tag

nxf2 
nxf2 
nxf2 
Panx 

Panx

prey

HA tag

Panx 
Panx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Panx 
C-terminal domain
sufficient binding region
unspecified
Panx 
C-terminal domain
necessary binding region
unspecified
nxf2 
NPC-binding domain
sufficient binding region
unspecified
nxf2 
UBA domain
necessary binding region
unspecified
nxf2 
UBA domain residues 811-814
mutation disrupting interaction
aa 811-814

IVEE to KRGG, coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0243162-1.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panoramix

bait

FLAG tag

nxf2 

Nxf2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0243162-10.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

bait

GFP tag

nxf2 
nxf2 
nxf2 
Panx 

Panoramix

prey

FLAG tag

Panx 
Panx 
Panx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Panx 
interaction domain
sufficient binding region
aa 263-446

coordinates relative to Panx-PA

Panx 
interaction domain
necessary binding region
aa 308-386

coordinates relative to Panx-PA

Panx 
predicted amphipathic alpha helix residues 328,331,332,335
mutation disrupting interaction
aa 328,331,332,335

coordinates relative to Panx-PA

nxf2 
UBA domain
sufficient binding region
aa 781-841

coordinates relative to nxf2-PA

nxf2 
UBA domain
necessary binding region
aa 781-841

coordinates relative to nxf2-PA

nxf2 
UBA domain residues 800-802,827-829
mutation disrupting interaction
aa 800-802,827-829

coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0243162-11.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panx

bait

His tag

Panx 
nxf2 

Nxf2

prey
nxf2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
UBA domain
sufficient binding region
aa 781-841

coordinates relative to nxf2-PA

Panx 
helix domain
sufficient binding region
aa 308-386

coordinates relative to Panx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0243162-12.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panx

neutral component
Panx 
Panx 

Panx

neutral component
Panx 
Panx 

Panx

neutral component
nxf2 
nxf2 

Nxf2

neutral component
nxf2 

Nxf2

neutral component
nxf2 
nxf2 

Nxf2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
UBA domain
sufficient binding region
aa 781-841

coordinates relative to nxf2-PA

Panx 
helix domain
sufficient binding region
aa 308-386

coordinates relative to Panx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

FBrf0243162-13.XL
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panx

neutral component
Panx 
Panx 

Panx

neutral component
Panx 
Panx 

Panx

neutral component
nxf2 

Nxf2

neutral component
nxf2 

Nxf2

neutral component
nxf2 

Nxf2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Panx 
helix domain
sufficient binding region
aa 263-446

coordinates relative to Panx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus and insect cell system.

FBrf0243162-3.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

bait

FLAG tag

Panx 

Panoramix

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0243162-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panoramix

bait
nxf2 

Nxf2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0243162-7.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panoramix

neutral component
Panx 

Panoramix

neutral component
Panx 
Panx 

Panoramix

neutral component
Panx 
nxf2 

Nxf2

neutral component
nxf2 

Nxf2

neutral component
nxf2 

Nxf2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Panx 
interaction domain
sufficient binding region
aa 263-446
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Panx, nxf2 and Nxt1 form a trimeric complex, captured as a series of three binary interactions.

Interaction in vitro; proteins produced as recombinant fusion proteins in a baculovirus and insect cell system.

FBrf0243284-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panx

bait

GFP tag

Panx 
nxf2 

Nxf2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Panx 
interaction domain
necessary binding region
aa 300-399

coordinates relative to Panx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell or nuclear extract of OSC cell line; bait produced from endogenous gene or transfected construct; prey produced from endogenous gene.

FBrf0243284-12.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

bait

FLAG tag

Panx 

Panx

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0243284-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panx

bait
nxf2 

Nxf2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0243284-7.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

bait

Myc tag

nxf2 
Panx 

Panx

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
UBA domain
necessary binding region
aa 777-841

coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0243284-8.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panx

bait
nxf2 

Nxf2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0244040-1.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panx

bait

GFP tag

nxf2 

dNxf2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0244040-11.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

bait
nxf2 
Panx 

Panx

prey
Panx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
UBA domain
sufficient binding region
aa 762-841

coordinates relative to nxf2-PA

Panx 
NIR domain
sufficient binding region
aa 315-343

coordinates relative to Panx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Kd = 3.2uM

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0244040-15.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

neutral component
nxf2 

dNxf2

neutral component
nxf2 
nxf2 

dNxf2

neutral component
nxf2 
Panx 

Panx

neutral component
Panx 
Panx 

Panx

neutral component
Panx 

Panx

neutral component
Panx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
UBA domain
sufficient binding region
aa 762-841

coordinates relative to nxf2-PA

Panx 
NIR domain
sufficient binding region
aa 315-343

coordinates relative to Panx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

FBrf0244040-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panx

bait

GFP tag

nxf2 

dNxf2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0244040-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

bait

Halo tag

Panx 

Panx

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0244040-33.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

neutral component
nxf2 
nxf2 

dNxf2

neutral component
nxf2 
nxf2 

dNxf2

neutral component
Panx 
Panx 

Panx

neutral component
Panx 

Panx

neutral component
Panx 
Panx 

Panx

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
NTF2 and UBA domains
sufficient binding region
aa 569-841

coordinates relative to nxf2-PA

Panx 
NIR domain
necessary binding region
aa 315-343

coordinates relative to Panx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

FBrf0244040-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panx

bait

GFP tag

Panx 
nxf2 

dNxf2

prey

Halo tag

nxf2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Panx 
NIR domain
necessary binding region
aa 315-343

coordinates relative to Panx-PA

nxf2 
UBA domain residues 826,827
mutation disrupting interaction
aa 826,827

F826A,I827A, coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0244040-5.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

bait

fused to lexA DNA-binding domain

nxf2 
nxf2 
nxf2 
nxf2 
Panx 

Panx

prey

fused to GAL4 activation domain

Panx 
Panx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
UBA domain
sufficient binding region
aa 762-841

coordinates relative to nxf2-PA

Panx 
NIR domain
sufficient binding region
aa 315-343

coordinates relative to Panx-PA

Panx 
NIR domain
necessary binding region
aa 315-345

coordinates relative to Panx-PA

nxf2 
UBA domain residue 823
mutation disrupting interaction
aa 823

L823A, coordinates relative to nxf2-PA

nxf2 
UBA domain residue 837
mutation decreasing interaction
aa 837

D837A, coordinates relative to nxf2-PA

nxf2 
UBA domain residues 826,827
mutation disrupting interaction
aa 826,827

F826A,I827A, coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast lexA-BD/GAL4-AD. In this assay, neither full-length nxf2 or a fragment of nxf2 spanning the NTF2 and UBA domains interacted with Panx while the nxf2 UBA domain alone did.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

FBrf0244040-8.Y3H
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

bait

fused to lexA DNA-binding domain

Panx 

Panx

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Three-hybrid system: yeast lexA-BD/GAL4-AD, Nxt1 was coexpressed as a bridging factor in a yeast three-hybrid assay with nxf2 as bait and Panx as prey. Full-length nxf2 binds to Panx in the presence of Nxt1.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

FBrf0248277-4.coIP.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Panx 

Panx

bait

GFP tag

nxf2 

Nxf2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from gene tagged at endogenous locus; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)