MBD-like, MTA1-like and Mi-2 proteins copurify in ion exchange chromatography and further co-elute on a gel filtration column with an apparent molecular mass of about 1 MDa.

Proteins endogenous to nuclear extract.

Nuclear extract was fractionated by ion exchange chromatography, and the MBD-like-containing fraction was subjected to molecular sieving by gel filtration chromatography.

Co-elution of Mi-2, MEP-1, HDAC1, MTA1-like and Caf1-55 in gel filtration captured as all possible binary interactions.

Source was nuclear extract of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.