FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
smo, krz
FlyBase ID
FBig0000095321
Interaction Network
Interactions Browser links
smo network
krz network
Reported Interactions
FBrf0213301-1.coIP.WB
Reference
Molnar et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

krz 

Krz

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged smo construct.

(Molnar et al., 2011)
FBrf0213301-2.coIP.WB
Reference
Molnar et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

krz 

Krz

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged smo and krz constructs.

(Molnar et al., 2011)
FBrf0217210-1.coIP.WB
Reference
Li et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
smo 

smo

bait

Myc tag

smo 
smo 
smo 
krz 

krz

prey

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal domain
sufficient binding region
aa 556-1035
smo 
C-terminal domain
necessary binding region
aa 556-1035
smo 
PKA,CK1 phosphorylation domain residues 667-746
mutation decreasing interaction
aa 667-746

S667D,S670D,S673D,S687D,S690D,S693D,S740D,S743D,S746D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2012)
FBrf0234792-3.coIP.WB
Reference
Zhang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

krz 

Krz

prey

HA tag

krz 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
krz 
regulatory region
mutation disrupting interaction
aa 427-470

"ΔR" construct, coordinates relative to krz-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2017)
FBrf0237567-1.coIP.WB
Reference
Jiang et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

krz 

Krz

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jiang et al., 2018)

Fusion of KKDE or FDNPVY motifs to smo increased the smo-krz interaction indicating that ER localization or endocytosis of smo increases binding to krz.

(Jiang et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)