FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sdt, par-6
FlyBase ID
FBig0000095331
Interaction Network
Interactions Browser links
sdt network
par-6 network
Reported Interactions
FBrf0180362-1.PD.WB
Reference
Wang et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait

GST tag

sdt 
sdt 
sdt 
par-6 

DmPar6

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sdt 
N-terminal region
sufficient binding region
aa 1-256

coordinates relative to sdt-PF

sdt 
ECR1 region residue V19
mutation disrupting interaction
aa 19

V19G, coordinates relative to sdt-PF

sdt 
ECR1 region residue D20
mutation disrupting interaction
aa 20

D20A, coordinates relative to sdt-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HEK293 cell extract.

(Wang et al., 2004)
FBrf0191891-2.Y2H
Reference
Kempkens et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait

fused to GAL4 DNA-binding domain

sdt 
par-6 

DmPar-6

prey

fused to GAL4 activation domain

par-6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sdt 
N-terminal region
sufficient binding region
aa 1-566

coordinates relative to sdt-PB

par-6 
PDZ domain
sufficient binding region
aa 156-261

coordinates relative to par-6-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kempkens et al., 2006)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Kempkens et al., 2006)
FBrf0191891-4.PD.WB
Reference
Kempkens et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait

GST tag

sdt 
par-6 

DmPar-6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sdt 
N-terminal region
sufficient binding region
aa 1-566

coordinates relative to sdt-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Kempkens et al., 2006)
FBrf0191891-6.PD.WB
Reference
Kempkens et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait

GST tag

sdt 
par-6 

DmPar-6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sdt 
N-terminal region
sufficient binding region
aa 1-566

coordinates relative to sdt-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.

(Kempkens et al., 2006)
FBrf0217811-4.coIP.MS
Reference
Neumüller et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GFP tag

sdt 

Sdt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a GFP-tagged par-6 construct in a parδ226 mutant background.

(Neumüller et al., 2012)

Prey endogenous to embryonic extract.

(Neumüller et al., 2012)
FBrf0234434-1.coIP.WB
Reference
Koch et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait

GFP tag

par-6 

PAR6

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transfected S2R+ cell extract; prey produced as a recombinant fusion protein in bacterial system.

(Koch et al., 2016)
FBrf0234434-2.coIP.WB
Reference
Koch et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait

GFP tag

sdt 
par-6 

PAR-6

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sdt 
ECR1 domain
necessary binding region
HREMAVDCP

"ΔECR1" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Koch et al., 2016)

This interaction was not observed using endogenous sdt and par-6 from embryonic lysates.

(Koch et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)