FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
hth, tsh
FlyBase ID
FBig0000095370
Interaction Network
Interactions Browser links
hth network
tsh network
Reported Interactions
FBrf0152003-3.PD.A
Reference
Bessa et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

Hth

bait

His tag

tsh 

Teashirt

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Bessa et al., 2002)

Prey derived from in vitro translation.

(Bessa et al., 2002)
FBrf0152003-5.coIP.WB
Reference
Bessa et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

Hth

bait
tsh 

Teashirt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait was endogenous to embryonic extract.

(Bessa et al., 2002)

Prey endogenous to embryonic extract.

(Bessa et al., 2002)
FBrf0216557-1.coIP.WB
Reference
Datta et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
hth 

HTH

bait

HA tag

tsh 

TSH

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Datta et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)