Bait endogenous to embryonic extract.

Prey endogenous to embryonic extract.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

Two-hybrid system: yeast Gal4-BD/GAL4-AD

Source was cell extract of SL2 cells; bait produced from transfected construct; prey produced from endogenous gene.

Positive control

Rbf did not coimmunoprecipitate with E2f1 in extract from embryos between stages 1 and 9.

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The white spot syndome virus proteins IE1 and WSV056 can bind to Rbf, displacing E2f. Consequently, E2f is activated and the G[[1]]/S transition is stimulated.

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

HGScore = 30.835206

The entire large-pocket, LP, domain of Rbf is required to efficiently bind the b isoform of E2f1 (E2f1-PD). The a isoform of E2f1 (E2f1-PA) binds efficiently to to both the small-pocket, SP, and C-terminal fragments of Rbf.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.