Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast
A mutated form of Cdc42 bearing an activating mutation (G12V) and a disruption of the prenylation motif (C189S).
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2R+ cell extract.
Positive control.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2R+ extract.
