FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cip4, WASp
FlyBase ID
FBig0000095405
Interaction Network
Interactions Browser links
Cip4 network
WASp network
Reported Interactions
FBrf0206177-3.coIP.WB
Reference
Leibfried et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Cip4 

Cip4

bait

GFP tag

WASp 

WASp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Leibfried et al., 2008)
FBrf0206177-4.coIP.WB
Reference
Leibfried et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
WASp 

WASp

bait

GFP tag

Cip4 

Cip4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Leibfried et al., 2008)
FBrf0209286-1.PD.WB
Reference
Fricke et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Cip4 

Cip4

bait

GST tag

WASp 

WASP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2R+ extract.

(Fricke et al., 2009)

Cip4 pulls downn WASp, SCAR and Hem, but not ena.

(Fricke et al., 2009)
FBrf0209286-10.coIP.WB
Reference
Fricke et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Cip4 

Cip4

bait

GFP tag

Cip4 
WASp 

WASP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cip4 
SH3 domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fricke et al., 2009)
FBrf0209286-4.coIP.WB
Reference
Fricke et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Cip4 

Cip4

bait
WASp 

WASP

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Fricke et al., 2009)
FBrf0209286-6.Y2H
Reference
Fricke et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
WASp 

WASP

bait

fused to GAL4 DNA-binding domain

Cip4 

Cip4

prey

fused to GAL4 activation domain

Cip4 
Cip4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cip4 
SH3 domain
sufficient binding region
Cip4 
SH3 domain
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Fricke et al., 2009)

Cip4 binds WASp and Abi, but not WAVE, in a two hybrid assay.

(Fricke et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Fricke et al., 2009)
FBrf0211075-2.PD.WB
Reference
Nahm et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cip4 

dCIP4

bait

GST tag

Cip4 
WASp 

Wsp

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cip4 
SH3 domain
unspecified role
aa 603

necessary: W603K mutation abolishes interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Nahm et al., 2010)

Prey expressed in transiently transfected HEK293T cells.

(Nahm et al., 2010)

GST pull down.

(Nahm et al., 2010)
FBrf0211075-3.PD.WB
Reference
Nahm et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
WASp 

Wsp

bait

GST tag

WASp 
Cip4 

dCIP4

prey

Myc tag

Cip4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
WASp 
PRD
unspecified role
aa 301-378
Cip4 
SH3 domain
unspecified role
aa 603

necessary: W603K mutation abolishes interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Nahm et al., 2010)

Prey expressed in transiently transfected HEK293T cells.

(Nahm et al., 2010)

GST pull down.

(Nahm et al., 2010)
FBrf0228613-1.FC.FM
Reference
Zobel et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cip4 

Cip4

neutral component

YFP tag, N-terminal fragment

Cip4 

Cip4

neutral component

YFP tag, N-terminal fragment

Cip4 

Cip4

neutral component

YFP tag, N-terminal fragment

WASp 

Wasp

neutral component

YFP tag, C-terminal fragment

WASp 

Wasp

neutral component

YFP tag, C-terminal fragment

WASp 

Wasp

neutral component

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cip4 interacts with WASp but not with Nost in this assay.

(Zobel et al., 2015)

Source was live S2 cells; proteins produced from transfected constructs.

(Zobel et al., 2015)

Positive control.

(Zobel et al., 2015)
FBrf0228613-3.PD.WB
Reference
Zobel et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Cip4 

Cip4

bait

GST tag

WASp 

Wasp

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cip4 interacts with WASp but only weakly with Nost in this assay.

(Zobel et al., 2015)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2R+ cell extract.

(Zobel et al., 2015)

Positive control.

(Zobel et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)