Rab11
GST tag
Evi5
GFP tag
S25N mutation is dominant negative
Q70L mutation locks protein in GTP-bound activated state
Bait expressed in stably transfected cells.
Prey expressed in transfected cells.
Evi5 binds more strongly to activated Rab11.
Mutation of Evi5 catalytic residue (R170A) does not inhibit binding to Rab11.
