FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
neur, Delta
FlyBase ID
FBig0000095953
Interaction Network
Interactions Browser links
neur network
Delta network
Reported Interactions
FBrf0187479-1.coIP.WB
Reference
Pitsouli and Delidakis, 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
neur 

Neur

bait

EGFP tag

neur 
Delta 

Dl

prey

V5-tag

Delta 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
neur 
interaction domain
sufficient binding region
unspecified

Binding still occurred when the protein lacked the Ring domain: Δneur

Delta 
intracellular proteolytic fragment
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line and larval disk/CNS complexes; bait produced from transfected construct; prey produced from transfected construct.

(Pitsouli and Delidakis, 2005)
FBrf0187479-2.coIP.WB
Reference
Pitsouli and Delidakis, 2005
Description
physical association
Assay
Collection
Source/Stage
imaginal disccentral nervous system / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
neur 

Neur

bait

EGFP tag

neur 
Delta 

Dl

prey

V5-tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
neur 
interaction domain
sufficient binding region
unspecified

Binding still occurred when the protein lacked the Ring domain: ΔRneur

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval disk/CNS complexes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Pitsouli and Delidakis, 2005)
FBrf0193514-1.coIP.WB
Reference
Commisso and Boulianne, 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
neur 

Neur

bait

V5 tag

neur 
neur 
neur 
Delta 

Dl

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
neur 
NHR1 domain
mutation disrupting interaction
aa 167

coordinates relative to neur-PA, G167E

neur 
NHR1 domain
sufficient binding region
aa 9-195

coordinates relative to neur-PC

neur 
NHR1 domain
necessary binding region
aa 1-172

coordinates relative to neur-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Commisso and Boulianne, 2007)
FBrf0201946-1.coIP.WB
Reference
Skwarek et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
neur 

Neur

bait

V5 tag

Delta 

Delta

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Skwarek et al., 2007)
FBrf0208547-3.PD.WB
Reference
Fontana and Posakony, 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Delta 

Dl

bait

GST tag

Delta 
neur 

Neur

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Delta 
intracellular domain
sufficient binding region
aa 619-833
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Fontana and Posakony, 2009)

E(spl)mα-BFM and E(spl)m4-BFM compete with neu binding to Dl.

(Fontana and Posakony, 2009)
FBrf0208547-4.PD.WB
Reference
Fontana and Posakony, 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
neur 

Neur

bait

GST tag

Delta 

Dl

prey

His tag

Delta 
Delta 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Delta 
intracellular domain
sufficient binding region
aa 619-833
Delta 
N motif
mutation disrupting interaction
aa 638-643

clustered alanine substitutions

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Fontana and Posakony, 2009)
FBrf0216896-2.coIP.WB
Reference
Daskalaki et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Delta 

Dl

bait

V5 tag

Delta 
neur 

Neur

prey

Myc tag

neur 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Delta 
region
necessary binding region
aa 680-687

\'Dli1\'

neur 
region
sufficient binding region

\'NeurΔR\'; lacks RING domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged Dl and neur constructs.

(Daskalaki et al., 2011)

Assay for docking site of neur protein; enzymatic domain removed.

(Daskalaki et al., 2011)
FBrf0216896-3.ENZ
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
neur 

Neur

enzyme
neur 

Neur

enzyme
Delta 

Dl

enzyme target

His tag

Delta 

Dl

enzyme target

His tag

Delta 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0220817-2.coIP.WB
Reference
Liu et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc
Participants
Corresponds to
Reported as
Role
Note
neur 

Neur

bait

V5 tag

neur 
Delta 

Delta

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
neur 
NHR1 domain
mutation disrupting interaction
aa 167

G167E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)