FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
gw, Not1
FlyBase ID
FBig0000095964
Interaction Network
Interactions Browser links
gw network
Not1 network
Reported Interactions
FBrf0216988-1.coIP.WB
Reference
Braun et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait
Not1 

NOT1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line.

(Braun et al., 2011)
FBrf0216988-2.coIP.WB
Reference
Braun et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

GFP tag

Not1 

NOT1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged gw and Not1 constructs.

(Braun et al., 2011)
FBrf0220586-1.coIP.WB
Reference
Huntzinger et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

GFP tag

gw 
gw 
gw 
gw 
Not1 

NOT1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gw 
Q-rich region and silencing domain
sufficient binding region
aa 713-1384
gw 
M2 region
mutation decreasing interaction

six Trp residues mutated to Ala (\'6xW\' mutant)

gw 
PAM2 motif
mutation decreasing interaction
aa 961

F961A

gw 
CIM-1 motif
mutation decreasing interaction
aa 870-875

S870A, R871A, W875A! IN7b. Comments concerning experimental data:

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huntzinger et al., 2013)

Mutation of the gw PAM2 and CIM-1 motifs decrease the interaction only when combined with mutations in the M2 region (Trp-to-Ala).

(Huntzinger et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)